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Profile Details
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USD 85 /hr
Hire Alexandre C.
Germany
USD 85 /hr

Biomedical Engineer | Microfluidics, Biosensors, Micro/Nanofabrication, Diagnostics

Profile Summary
Subject Matter Expertise
Services
Writing Technical Writing, Translation
Research Technology Scouting, Fact Checking, Gray Literature Search, Scientific and Technical Research
Consulting Scientific and Technical Consulting, Manufacturing Consulting
Product Development Recipe Development, Material Sourcing, Product Validation, Manufacturing, Concept Development, Product Launch Support, Prototyping, Reverse Engineering, Device Fabrication
Work Experience

MSCA Postdoctoral Fellow

Humboldt-Universität zu Berlin

October 2023 - Present

PhD Research Fellow

International Iberian Nanotechnology Laboratory

December 2013 - September 2023

Research fellow

Instituto de Engenharia de Sistemas e Computadores Microsistemas e Nanotecnologias

December 2012 - October 2013

Research intern

Universidade de São Paulo Faculdade de Filosofia Ciências e Letras de Ribeirão Preto

January 2008 - June 2008

Education

Ph.D. Program in Biomedical Engineering (Bioengineering Department)

Universidade de Lisboa Instituto Superior Técnico

March 2014 - October 2018

M.Sc. in Biomedical engineering (Bioengineering Department)

Instituto Superior Técnico

October 2003 - December 2012

International Exchange Student (Genetics Department)

Universidade de São Paulo Faculdade de Filosofia Ciências e Letras de Ribeirão Preto

August 2007 - October 2008

Certifications
  • Certification details not provided.
Publications
JOURNAL ARTICLE
Alexandre Chícharo, Zdenek Kaspar, Tatiana G. Rappoport, Ajinkya Punjal, Chun-Da Liao, Pieter De Beule, Jérôme Borme, Nuno M. R. Peres, Pedro Alpuim (2025). Broadband high-performance terahertz polarizers by nanoimprint lithography for advanced applications . Optics Express.
Chícharo, A., Kaspar, Z., Rappoport, T.G., Punjal, A., Liao, C.-D., de Beule, P., Borme, J., Peres, N.M.R., Alpuim, P.(2025). Broadband high-performance terahertz polarizers by nanoimprint lithography for advanced applications . Optics Express. 33. (17). p. 36682-36697.
Chícharo, A., Teixeira, A., Moreira, D., Relvas, M.S., Aranda, M., Douglas, T., Borme, J., Nieder, J.B., Diéguez, L., Abalde-Cela, S.(2025). Precision-Engineered Plasmonic Nanostar Arrays for High-Performance SERS Sensing . Advanced Optical Materials.
Beatriz N. L. Costa, Filipe Camarneiro, Ana Marote, Catarina Barbosa, Carlos Vedor, Diogo Tomé, Filipa J. Costa, Marta S. Dias, Joana Correia, Joel Pires, et al. (2024). Functionalized Nanodiamonds for Targeted Neuronal Electromagnetic Signal Detection . ACS Applied Materials & Interfaces.
Teixeira, A., Sousa-Silva, M., Chícharo, A., Oliveira, K., Moura, A., Carneiro, A., Piairo, P., Águas, H., Sampaio-Marques, B., Castro, I., et al.(2024). Isolation of acute myeloid leukemia blasts from blood using a microfluidic device . Analyst. 149. (10). p. 2812-2825.
Alexandra Teixeira, Maria Sousa-Silva, Alexandre Chícharo, Kevin Oliveira, André Moura, Adriana Carneiro, Paulina Piairo, Hugo Águas, Belém Sampaio-Marques, Isabel Castro, et al. (2024). Isolation of acute myeloid leukemia blasts from blood using a microfluidic device . The Analyst.
Costa, B.N.L., Camarneiro, F., Marote, A., Barbosa, C., Vedor, C., Tomé, D., Costa, F.J., Dias, M.S., Correia, J., Pires, J., et al.(2024). Functionalized Nanodiamonds for Targeted Neuronal Electromagnetic Signal Detection . ACS Applied Materials and Interfaces. 16. (44). p. 60828-60841.
Ahmed Y.F. Mahmoud, Alexandra Teixeira, Marta Aranda, Maria S. Relvas, Sergio Quintero, Maria Sousa-Silva, Alexandre Chícharo, Mengkun Chen, Mohadeseh Hashemi, Jason B. King, et al. (2023). Will data analytics revolution finally bring SERS to the clinic? . TrAC Trends in Analytical Chemistry.
Kevin Oliveira, Alexandra Teixeira, José M. Fernandes, Cláudia Lopes, Alexandre Chícharo, Paulina Piairo, Lei Wu, Laura Rodríguez‐Lorenzo, Lorena Diéguez, Sara Abalde‐Cela (2023). Multiplex SERS Phenotyping of Single Cancer Cells in Microdroplets . Advanced Optical Materials.
Oliveira, K., Teixeira, A., Fernandes, J.M., Lopes, C., Chícharo, A., Piairo, P., Wu, L., Rodríguez-Lorenzo, L., Diéguez, L., Abalde-Cela, S.(2023). Multiplex SERS Phenotyping of Single Cancer Cells in Microdroplets . Advanced Optical Materials. 11. (1).
Mahmoud, A.Y.F., Teixeira, A., Aranda, M., Relvas, M.S., Quintero, S., Sousa-Silva, M., Chícharo, A., Chen, M., Hashemi, M., King, J.B., et al.(2023). Will data analytics revolution finally bring SERS to the clinic? . Trac Trends in Analytical Chemistry. 169.
Adriana Carneiro, Paulina Piairo, Alexandra Teixeira, Dylan Ferreira, Sofia Cotton, Carolina Rodrigues, Alexandre Chícharo, Sara Abalde-Cela, Lúcio Lara Santos, Luís Lima, et al.(2022). Discriminating Epithelial to Mesenchymal Transition Phenotypes in Circulating Tumor Cells Isolated from Advanced Gastrointestinal Cancer Patients . Cells. 11. (3). p. 376. {MDPI} {AG}
Adriana Carneiro, Paulina Piairo, Alexandra Teixeira, Dylan Ferreira, Sofia Cotton, Carolina Rodrigues, Alexandre Chícharo, Sara Abalde-Cela, Lúcio Lara Santos, Luís Lima, et al. (2022). Discriminating Epithelial to Mesenchymal Transition Phenotypes in Circulating Tumor Cells Isolated from Advanced Gastrointestinal Cancer Patients . Cells.
Carneiro, A., Piairo, P., Teixeira, A., Ferreira, D., Cotton, S., Rodrigues, C., Chícharo, A., Abalde-Cela, S., Santos, L.L., Lima, L., et al.(2022). Discriminating Epithelial to Mesenchymal Transition Phenotypes in Circulating Tumor Cells Isolated from Advanced Gastrointestinal Cancer Patients . Cells. 11. (3).
Cláudia Lopes, Paulina Piairo, Alexandre Chícharo, Sara Abalde-Cela, Liliana R. Pires, Patrícia Corredeira, Patrícia Alves, Laura Muinelo-Romay, Luís Costa, Lorena Diéguez (2021). HER2 Expression in Circulating Tumour Cells Isolated from Metastatic Breast Cancer Patients Using a Size-Based Microfluidic Device . Cancers.
Cláudia Lopes, Paulina Piairo, Alexandre Chícharo, Sara Abalde-Cela, Liliana R. Pires, Patrícia Corredeira, Patrícia Alves, Laura Muinelo-Romay, Luís Costa, Lorena Diéguez (2021). HER2 Expression in Circulating Tumour Cells Isolated from Metastatic Breast Cancer Patients Using a Size-Based Microfluidic Device . Cancers.
Rúben F. Santos, Bruno M. C. Oliveira, Alexandre Chícharo, Pedro Alpuim, Paulo J. Ferreira, Sónia Simões, Filomena Viana, Manuel F. Vieira(2021). Seedless Cu Electroplating on Co-W Thin Films in Low pH Electrolyte: Early Stages of Formation . Nanomaterials. 11. (8). p. 1914. {MDPI} {AG}
Rúben F. Santos, Bruno M. C. Oliveira, Alexandre Chícharo, Pedro Alpuim, Paulo J. Ferreira, Sónia Simões, Filomena Viana, Manuel F. Vieira (2021). Seedless Cu Electroplating on Co-W Thin Films in Low pH Electrolyte: Early Stages of Formation . Nanomaterials.
Santos, R.F., Oliveira, B.M.C., Chícharo, A., Alpuim, P., Ferreira, P.J., Simões, S., Viana, F., Vieira, M.F.(2021). Seedless Cu Electroplating on Co-W Thin Films in Low pH Electrolyte: Early Stages of Formation . Nanomaterials. 11. (8).
Lopes, C., Piairo, P., Chícharo, A., Abalde-Cela, S., Pires, L.R., Corredeira, P., Alves, P., Muinelo-Romay, L., Costa, L., Diéguez, L.(2021). Her2 expression in circulating tumour cells isolated from metastatic breast cancer patients using a size-based microfluidic device . Cancers. 13. (17).
Alexandre Chicharo, Patrícia D. Cabral, Telma Domingues, George Machado, Jr., Fátima Cerqueira, Elisabete Fernandes, Emília Athayde, Pedro Alpuim, Jérôme Borme(2020). Clean-Room Lithographical Processes for the Fabrication of Graphene Biosensors . Materials. 13. (24). p. 5728. {MDPI} {AG}
Alexandre Chicharo, Patrícia D. Cabral, Telma Domingues, George Machado, Fátima Cerqueira, Elisabete Fernandes, Emília Athayde, Pedro Alpuim, Jérôme Borme (2020). Clean-Room Lithographical Processes for the Fabrication of Graphene Biosensors . Materials.
Cabral, P.D., Domingues, T., Machado, G., Chicharo, A., Cerqueira, F., Fernandes, E., Athayde, E., Alpuim, P., Borme, J.(2020). Clean-room lithographical processes for the fabrication of graphene biosensors . Materials. 13. (24). p. 1-23.
Chícharo, Alexandre, Martins, Marco, Barnsley, Lester C., Taouallah, Amal, Fernandes, João, Silva, Bruno F. B., Cardoso, Susana, Diéguez, Lorena, Espiña, Begoña, Freitas, Paulo P.(2018). Enhanced magnetic microcytometer with 3D flow focusing for cell enumeration . Lab on a Chip. 18. (17). p. 2593-2603.
Alexandre Chícharo, Marco Martins, Lester C. Barnsley, Amal Taouallah, João Fernandes, Bruno F. B. Silva, Susana Cardoso, Lorena Diéguez, Begoña Espiña, Paulo P. Freitas(2018). Enhanced magnetic microcytometer with 3D flow focusing for cell enumeration . Lab on a Chip. 18. (17). p. 2593--2603. Royal Society of Chemistry ({RSC})
Chícharo, A., Martins, M., Barnsley, L.C., Taouallah, A., Fernandes, J., Silva, B.F.B., Cardoso, S., Diéguez, L., Espiña, B., Freitas, P.P.(2018). Enhanced magnetic microcytometer with 3D flow focusing for cell enumeration . Lab on a Chip. 18. (17). p. 2593-2603.
A. Chicharo, L. C. Barnsley, M. Martins, S. Cardoso, L. Di, x00E,, guez,, B. Espi, x00F,, P. P. Freitas(2018). Custom Magnet Design for a Multi-Channel Magnetic Microcytometer . IEEE Transactions on Magnetics. p. 1-5.
Chicharo, A., Barnsley, L.C., Martins, M., Cardoso, S., Dieguez, L., Espina, B., Freitas, P.P.(2018). Custom magnet design for a multi-channel magnetic microcytometer . IEEE Transactions on Magnetics. 54. (11).
Cardoso, S., Leitao, D. C., Dias, T. M., Valadeiro, J., Silva, M. D., Chicharo, A., Silverio, V., Gaspar, J., Freitas, P. P.(2017). Challenges and trends in magnetic sensor integration with microfluidics for biomedical applications . Journal of Physics D-Applied Physics. 50. (21).
Cardoso, S., Leitao, D.C., Dias, T.M., Valadeiro, J., Silva, M.D., Chicharo, A., Silverio, V., Gaspar, J., Freitas, P.P.(2017). Challenges and trends in magnetic sensor integration with microfluidics for biomedical applications . Journal of Physics D: Applied Physics. 50. (21).
Chicharo, A., Cardoso, F., Cardoso, S., Freitas, P.J.P.(2015). Real-time monitoring of magnetic nanoparticles diffusion in lateral flow microporous membrane using spin valve sensors . IEEE Transactions on Magnetics. 51. (1).
Chicharo, Alexandre, Cardoso, Filipe, Cardoso, Susana, Freitas, Paulo J. P.(2015). Real-Time Monitoring of Magnetic Nanoparticles Diffusion in Lateral Flow Microporous Membrane Using Spin Valve Sensors . Ieee Transactions on Magnetics. 51. (1).
Vila, A., Martins, V.C., Chícharo, A., Rodriguez-Abreu, C., Fernandes, A.C., Cardoso, F.A., Cardoso, S., Rivas, J., Freitas, P.(2014). Customized design of magnetic beads for dynamic magnetoresistive cytometry . IEEE Transactions on Magnetics. 50. (11).
Chicharo, Alexandre, Cardoso, Filipe, Cardoso, Susana, Freitas, Paulo P.(2014). Dynamical Detection of Magnetic Nanoparticles in Paper Microfluidics With Spin Valve Sensors for Point-of-Care Applications . Ieee Transactions on Magnetics. 50. (11).
Chicharo, A., Cardoso, F., Cardoso, S., Freitas, P.P.(2014). Dynamical detection of magnetic nanoparticles in paper microfluidics with spin valve sensors for point-of-care applications . IEEE Transactions on Magnetics. 50. (11).
Chicharo, Alexandre, Vila, Ana, Martins, Veronica C., Rodriguez-Abreu, Carlos, Fernandes, Ana Carolina, Cardoso, Filipe A., Cardoso, Susana, Rivas, Jose, Freitas, Paulo(2014). Customized Design of Magnetic Beads for Dynamic Magnetoresistive Cytometry . Ieee Transactions on Magnetics. 50. (11).
PATENT
BOOK CHAPTER
Chícharo, A., Caetano, D.M., Cardoso, S., Freitas, P.(2022). Evolution in Automatized Detection of Cells: Advances in Magnetic Microcytometers for Cancer Cells . Advances in Experimental Medicine and Biology. 1379. p. 413-444.
Alexandre Chícharo, Diogo Miguel Caetano, Susana Cardoso, Paulo Freitas (2022). Evolution in Automatized Detection of Cells: Advances in Magnetic Microcytometers for Cancer Cells . Advances in Experimental Medicine and Biology.
Freitas, P.P., Martins, V.C., Cardoso, F.A., Fernandes, E., Sobrino, T., Castillo, J., Chicharo, A., Abal, M., Lopez, R.L., Dias, T.S., et al.(2017). Spintronic Biochips: From the Laboratory to Pre-Clinical Applications . Nanomagnetism Applications and Perspectives. p. 165-199.
OTHER
Chícharo, A., Kaspar, Z., Rappoport, T.G., Punjal, A., Liao, C.-D., de Beule, P., Borme, J., Peres, N.M.R., Alpuim, P.(2021). Broadband High-Performance Terahertz Polarizers by Nanoimprint Lithography for Advanced Applications . Arxiv.
Alexandre Chicharo, Paulo P. Freitas, Veronica C. Martins, Felipe A. Cardoso, Elisabete Fernandes, Tomas Sobrino, Jose Castillo, Miguel Abal, Rafael Lopez‐Lopez, Tomas S. Dias, et al. (2017). Spintronic Biochips . Nanomagnetism: Applications and Perspectives.
DISSERTATION THESIS
Design and optimization of a magnetic microcytometer for cell detection and enumeration @phdthesis { author = {Chícharo, Alexandre}, title = {Design and optimization of a magnetic microcytometer for cell detection and enumeration}, year = {2018}, month = {oct}, school = {Universidade de Lisboa Instituto Superior Técnico} }. Universidade de Lisboa Instituto Superior Técnico.
CONFERENCE POSTER
Detection of pathogenic bacteria Escherichia coli in water samples using a lab-on-a-chip magnetoresistive flow cytometer @misc { author = {Marília B. Santos and Chícharo, Alexandre and Amal Taouallah and Vania Vilas-Boas and Marco Martins and João Piteira and Lorena Diéguez and Susana Freitas and Begoña Espiña}, title = {Detection of pathogenic bacteria Escherichia coli in water samples using a lab-on-a-chip magnetoresistive flow cytometer}, year = {2018}, month = {jun} }. Biosensors 2018.
Magnetoresistive flow cytometer for the detection of pathogenic bacteria Escherichia coli in water samples @misc { author = {Marília B. Santos and Chícharo, Alexandre and Amal Taouallah and Vania Vilas-Boas and Marco Martins and João Piteira and Lorena Diéguez and et al.}, title = {Magnetoresistive flow cytometer for the detection of pathogenic bacteria Escherichia coli in water samples}, year = {2017}, month = {nov} }. Biosensing Cambridge UK 2017.
Magnetic flow cytometer for circulating tumor cell quantification @misc { author = {Chícharo, Alexandre and Marco Martins and Susana Cardoso and Begoña Espiña and Lorena Diéguez and Paulo P. Freitas}, title = {Magnetic flow cytometer for circulating tumor cell quantification}, year = {2016}, month = {may} }. Biosensors 2016.
Magnetic flow cytometer biochip for Circulating Tumour Cell enumeration @misc { author = {Chícharo, Alexandre and Ana Vila and Carlos Rodriguez-Abreu and Clotilde Costa and Susana Cardoso and Paulo P. Freitas}, title = {Magnetic flow cytometer biochip for Circulating Tumour Cell enumeration}, year = {2015}, month = {may} }. 4th International Conference on Bio-Sensing Technology.
Magnetic fluid monitoring in porous medium using Spin Valves for Point-of-care solutions @misc { author = {Chícharo, Alexandre and Filipe Cardoso and Susana Cardoso and Paulo P. Freitas}, title = {Magnetic fluid monitoring in porous medium using Spin Valves for Point-of-care solutions}, year = {2014}, month = {jul} }. EMSA 2014.
Dynamical detection of magnetic nanoparticles in paper microfluidics with spin valve sensors @misc { author = {Chícharo, Alexandre and Filipe Cardoso and Susana Cardoso and Paulo P. Freitas}, title = {Dynamical detection of magnetic nanoparticles in paper microfluidics with spin valve sensors}, year = {2014}, month = {may} }.