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Hire Alexandre B.

Brazil

USD 100 /hr

PhD with extensive background and expertise in 3D cell cultures and bioimage analysis

Profile Summary

RESEARCH EXPERIENCE Rice University Fulbright distinguished chair 01/2023-05/2023 - Teaching Fundamentals of Microscopy and Computational Bioimage Analysis with Python and ImageJ - Research on the enteric neuron system development in zebrafish - Implement bioimage analysis workflows in Python for cell tracking Visiting Professor 01/2023-04/2014 - Research on the enteric neuron system development in zebrafish University of São Paulo – Brazil Associate Professor, Institute of Chemistry, Department of Biochemistry - 11/2022-present Assistant Professor, Institute of Chemistry, Department of Biochemistry - xxxxxxxxxxxxxxx -Lead Research program focused on cell cycle regulation in the context of the extracellular matrix in normal and tumor cells -Design CRISPR/Cas9 genome wide screens and image-based assays using 2D and 3D (organoid) cell cultures -Execute data collection, analysis, and interpretation. Write reports and papers -Hands-on tasks in establishing bioimage analysis workflows using Python scripts and packages -Mentor of PhD candidates and postdoc fellows -Teach biochemistry, molecular biology and Bioimage Analysis courses -Give talks in international conferences and institutions -Associate Editor of Biochemical Society Transactions (UK) since 2019 FAPESP Young Investigator Award -Instituto Serrapilheira fellow (2017-2019) -Affiliate member of the Brazilian Academy of Sciences Lawrence Berkeley National Laboratory – Berkeley-CA USA- Mina Bissell laboratory Postdoctoral fellow in cancer biology, Department of DNA damage responses and cancer biology (2010-2014) -Led a research project focused on unveiling the role of nuclear actin in cell proliferation in normal tissues and tumors (publication: http://dx.doi.org/10.1016/j.celrep.2017.05.050) -Implemented computational microscopy analysis workflows in ImageJ -Developed 3D cell culture assays for studying cell growth/dormancy of normal and breast cancer cells -Performed time-lapse confocal microscopy, advanced microscopy assays (FRAP, FLIP and FRET) and image analysis -Presented results at international conferences -Designed experiments, executed data synthesis, collection, analysis, and interpretation. Wrote reports and papers -Co-authored important papers in the field of breast cancer and mammary gland biology by contributing with 3D assays and quantitative fluorescence microscopy -Co-mentored undergrad and grad students EDUCATION University of Campinas - Brazil Ph.D., Cell and structural Biology - 2010 Τhesis: Involvement of matrix metalloproteinases in the rodent ventral prostate development and regression University of Campinas - Brazil M.Sc., Cell and structural Biology - 2006 Dissertation: Dynamics of epithelium and gelatinase activity in rat ventral prostate during the first week of post-natal development State University of São Paulo - Brazil Bachelor of Biological Sciences - 2004 Computational Bioimage Analysis Skills Technical Expertise• Python-based bioimage analysis with NumPy, Scikit-image, and Matplotlib •Quantitative analysis of microscopy data for morphological and fluorescence measurements •Custom bioimage processing workflows from raw data to visualization •Implementation of deep learning models (Stardist, Cellpose) for cell segmentation •Training of custom neural networks for specialized bioimage applications •Pytorch and Fastai for image classification •Integration of AI solutions to automate microscopy analysis workflows •Development of automated pipelines for microscopy data •Advanced visualization methods including "Superplots" for scientific communication •Extraction of insights from multi-dimensional image datasets •Optimized Python environments (Miniconda, Google Colab) for reproducible analysis GitHub page: https://github.com/brunicardoso OTHER SKILLS Basic bioinformatics tools and competency in analysis of scRNA seq using Python and R. Languages English: fluent; Portuguese: native

Subject Matter Expertise

• ☆ Computational Biology
• ☆ Molecular Biology
• ☆ Genetics
• ☆ Cell Biology
• ☆ Microscopy
• ☆ Light Microscopy
• ☆ Fluorescence Microscopy
• ☆ 3D Cell Culture
• ☆ Python
• ☆ R

Services

• Writing
• Research
• Consulting
• Data & AI

Writing Technical Writing

Research Meta-Research, Feasibility Study, Fact Checking, Scientific and Technical Research, Systematic Literature Review

Consulting Scientific and Technical Consulting

Data & AI Predictive Modeling, Statistical Analysis, Image Processing, Image Analysis, Algorithm Design-ML, Data Visualization, Big Data Analytics, Data Mining, Data Cleaning, Data Processing, Data Insights

Work Experience

Associate Professor

Universidade de São Paulo Instituto de Química

April 2014 - Present

Fulbright Distinguished Chair

Rice University

January 2023 - May 2024

Education

postdoc (Cancer and DNA damage)

E O Lawrence Berkeley National Laboratory

May 2010 - February 2014

PhD (Cell Biology)

Universidade Estadual de Campinas

March 2006 - March 2010

Masters (Cell Biology)

Universidade Estadual de Campinas

March 2004 - March 2006

B.S. (Biology)

Universidade Estadual Paulista Júlio de Mesquita Filho Câmpus de São José do Rio Preto Instituto de Biociências Letras e Ciências Exatas

March 1999 - January 2004

Certifications

• PyTorch for Deep Learning

  deeplearning.ai

  https://learn.deeplearning.ai/certificates/759237d5-8f2c-404d-b116-5fa051e84050?usp=sharing

  January 2026 - Present

Publications

JOURNAL ARTICLE

Alexandre Bruni-Cardoso, Ana Maria Rodrigues, Ana Paula Zen Petisco Fiore, Gabriela D. A. Guardia, Rebeka Tomasin, André Azevedo Reis Teixeira, Ricardo Jose Giordano, Deborah Schechtman, Michele Pagano, Pedro A. F. Galante (2024). Identification of a novel alternative splicing isoform of the Hippo kinase STK3/MST2 with impaired kinase and cell growth suppressing activities . Oncogene.

Antonio Carlos Manucci, Ana Paula Zen Petisco Fiore, Giovani Luiz Genesi, Alexandre Bruni‐Cardoso (2024). The basement membrane regulates the cellular localization and the cytoplasmic interactome of Yes‐Associated Protein (YAP) in mammary epithelial cells . Journal of Cellular Biochemistry.

Alexandre Bruni-Cardoso, Rebeka Tomasin, Ana Maria Rodrigues, Antonio Carlos Manucci (2023). A molecular landscape of quiescence and proliferation highlights the role of Pten in mammary gland acinogenesis . Journal of Cell Science.

Alexandre Bruni-Cardoso, Rebeka Tomasin (2022). The role of cellular quiescence in cancer – beyond a quiet passenger . Journal of Cell Science.

Isaias Glezer, Alexandre Bruni‐Cardoso, Deborah Schechtman, Bettina Malnic (2021). Viral infection and smell loss: The case of COVID‐19 . Journal of Neurochemistry.

M.S. Baptista, M.J.M. Alves, G.M. Arantes, H.A. Armelin, O. Augusto, R.L. Baldini, D.S. Basseres, E.J.H. Bechara, A. Bruni-Cardoso, H. Chaimovich, et al.(2019). Where do we aspire to publish? A position paper on scientific communication in biochemistry and molecular biology . Brazilian Journal of Medical and Biological Research. 52. (9). {FapUNIFESP} ({SciELO})

PREPRINT

Alexandre Bruni-Cardoso, Ana Rodrigues, Ana Fiore, Gabriela Guardia, Rebeka Tomasin, André Teixeira, Ricardo Giordano, Michele Pagano, Pedro Galante (2024). Identification of a novel alternative splicing isoform of the Hippo kinase STK3/MST2 with impaired tumor suppressor activities .

Alexandre Bruni-Cardoso, Rebeka Tomasin, Ana Maria Rodrigues, Antonio Carlos Manucci (2023). Pten is a disconnecting node in the molecular landscape of the proliferation/quiescence decision during mammary gland acinogenesis .

Alexandre Bruni-Cardoso, Ana Paula Zen Petisco Fiore, Ana Maria Rodrigues da Silva, Helder Veras Ribeiro Filho, Antonio Carlos Manucci, Pedro de Freitas Ribeiro, Mayara Carolinne Silva Botelho, Paulo Sergio Lopes de Oliveira, Michele Pagano (2021). Extracellular matrix stiffness regulates degradation of the Hippo kinase MST2 via SCFβTrCP .