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Hire Dr. Yamunadevi S.

United States

USD 30 /hr

Freelance consultant with 10+years of research experince.

Profile Summary

Research Scientist with expertise in Gene editing, Gene therapy, Super resolution microscopy, Biophysical methods, Membrane biology and Biochemistry. My key strengths are, Viral vectors: Lentiviral production and AAV9 (Adeno-associated virus 9) Cell culture: Tissue homogenization, cell fractionation, HepG2,Fibroblasts, human brain microvascular endothelial cells(HBMECs), primary smooth muscle cells, iPSC, THP-1 cells, PBMCs, HEK293 cells and cos-1 cells (African green monkey kidney cells). Imaging: Confocal microscopy, Total internal reflection microscopy, Live cell imaging, Time-lapse microscopy. Molecular Biology: Cloning, Plasmid preparation, ligation, transformation, Genomic DNA extraction, RT-PCR, PCR, site-directed mutagenesis, CRISPR-CAS9, siRNA. Biochemistry: Protein purification, column chromatography, membrane filtration, dialysis, SDS-PAGE, Western blot, bacterial protein expression. In-vivo models: Mouse models. Liposomes: Supported planar lipid bilayers, Giant unilamellar vesicles (GUVs), Large unilamellar vesicles (Luvs), Proteoliposomes. Bioinformatics skills: MATLAB, R, Perl, UNIX, LINUX, Windows, Eclipse (IDE), Galaxy, Gene expression analysis, 3D structural modeling, Database searching and data mining.

Subject Matter Expertise

• ★ Biochemistry
• ★ Neuroscience
• ☆ Separation & Purification
• ☆ Microencapsulation
• ☆ Peptide Synthesis
• ☆ Biology/Life Sciences
• ☆ Molecular Biology
• ☆ PCR
• ☆ Kinases
• ☆ Biophysics
• ☆ Microscopy
• ☆ Data Analysis
• ☆ Bioinformatics
• ☆ Protein/Enzyme Engineering
• ☆ Blotting
• ☆ German
• ☆ Hepatitis

Services

• Writing
• Research
• Consulting
• Data & AI
• Product Development

Writing Technical Writing, Copywriting

Research Market Research, User Research, Meta-Research, Feasibility Study, Fact Checking, Gap Analysis, Gray Literature Search, Systematic Literature Review, Secondary Data Collection

Consulting Digital Strategy Consulting, Scientific and Technical Consulting

Data & AI Predictive Modeling, Statistical Analysis, Data Visualization, Big Data Analytics, Data Processing

Product Development Formulation, Product Evaluation, Concept Development

Work Experience

Postdoctoral fellow

Harvard Medical School

September 2016 - Present

PhD

Max Planck Institute for Intelligent Systems

July 2010 - January 2014

PhD Student

Max Planck Institute for Intelligent Systems

June 2010 - January 2014

Junior Research Fellow

Indian institute of science

June 2008 - June 2010

Education

PhD

Max Planck Institute for Intelligent systems

July 2010 - January 2014

Diploma in Bioinformatics

Madurai Kamaraj University

August 2007 - May 2008

M.Sc Zoology(Specialization in Biotechnology)

Madurai Kamaraj University

June 2005 - May 2007

B.Sc Zoology(Specialization in Biotechnology)

Madurai Kamaraj University

June 2002 - April 2005

Certifications

• Certification details not provided.

Publications

JOURNAL ARTICLE

Yamunadevi Subburaj, Ikhwan Sudji, Nataliya Frenkel, Ana García-Sáez, Michael Wink(2015). Membrane Disintegration Caused by the Steroid Saponin Digitonin Is Related to the Presence of Cholesterol . Molecules. 20. (11). p. 20146--20160. {MDPI} {AG}

Yamunadevi Subburaj, Katia Cosentino, Markus Axmann, Esteban Pedrueza-Villalmanzo, Eduard Hermann, Stephanie Bleicken, Joachim Spatz, Ana J. García-Sáez(2015). Bax monomers form dimer units in the membrane that further self-assemble into multiple oligomeric species . Nature Communications. 6. p. 8042. Nature Publishing Group

E. Hermann, S. Bleicken, Y. Subburaj, A. J. Garcia-Saez(2014). Automated analysis of giant unilamellar vesicles using circular Hough transformation . Bioinformatics. 30. (12). p. 1747--1754. Oxford University Press ({OUP})

Yamunadevi Subburaj, Uris Ros, Eduard Hermann, Rudi Tong, Ana J. García-Sáez(2014). Toxicity of an $\upalpha$-Pore-forming Toxin Depends on the Assembly Mechanism on the Target Membrane as Revealed by Single Molecule Imaging . Journal of Biological Chemistry. 290. (8). p. 4856--4865. American Society for Biochemistry {\&} Molecular Biology ({ASBMB})

Yamunadevi Subburaj, Ana J. García Sáez(2013). Single Particle Tracking to Characterize the Mechanism of Pore Formation by {EquinatoxinII} . Biophysical Journal. 104. (2). p. 177a. Elsevier {BV}

Yamunadevi Subburaj, Joseph D. Unsay, Katia Cosentino, Ana J. García-Sáez(2013). Cardiolipin Effects on Membrane Structure and Dynamics . Langmuir. 29. (51). p. 15878--15887. American Chemical Society ({ACS})

Subburaj Yamunadevi, Govindarajan Sudha, Nidhi Tyagi, Saumitra Das, Narayanaswamy Srinivasan(2012). Structural and molecular basis of interaction of {HCV} non-structural protein 5A with human casein kinase 1$\upalpha$ and {PKR} . {BMC} Structural Biology. 12. (1). p. 28. Springer Science $\mathplus$ Business Media

R. Rakshambikai, S. Yamunadevi, K. Anamika, N. Tyagi, N. Srinivasan(2012). Repertoire of Protein Kinases Encoded in the Genome {ofTakifugu} rubripes . Comparative and Functional Genomics. 2012. p. 1--12. Hindawi Publishing Corporation

V. Malshetty, K. Kurthkoti, A. China, B. Mallick, S. Yamunadevi, P. B. Sang, N. Srinivasan, V. Nagaraja, U. Varshney(2010). Novel insertion and deletion mutants of {RpoB} that render Mycobacterium smegmatis {RNA} polymerase resistant to rifampicin-mediated inhibition of transcription . Microbiology. 156. (5). p. 1565--1573. Microbiology Society

OTHER

Yamunadevi Subburaj, Monika Zelman-Femiak, Ana J. García-Sáez(2012). Fluorescence Correlation Spectroscopy to Study Membrane Organization and Interactions . 241--269Springer Science $\mathplus$ Business Media

Yamunadevi Subburaj, Raquel Salvador Gallego, Ana J. García-Sáez (2000). Membrane Dynamics: Fluorescence Spectroscopy . Encyclopedia of Analytical Chemistry.