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Hire Dr. Thokhir Basha S.

India

Molecular Biologist, Biotechnologist, Chemical Biologist, nano biologist, protein purification with 8 years of experienc

Profile Summary

I worked in various projects in different research areas related to microbiology, molecular biology, cancer research, protein purification, and analytical process development for the microbial biotransformation products. In my doctoral degree mainly focused on the exploitation of chemical libraries for screening of anti-infective potentiality in various disease conditions and development of stable, biocompatible drug delivery system for the anti-infective agents. Skills acquired during my previous experience are • Animal Cell culture: Handling and maintenance of cell lines, Screening of compounds for cytotoxic activity (MTT & SRB assay) • Molecular Biology: Polymerase chain reaction, Restriction analysis of Plasmid DNA, Plasmid isolation in small and large scale, Agarose Gel Electrophoresis, Isolation of gDNA and RNA, DNA binding studies (RED assay etc.) • Protein work: Recombinant protein expression, Protein purification using FPLC, Enzyme assays and inhibitor assays, and Native PAGE, Western Blot • Microbial work: Screening for antimicrobial and antifungal activities of a compound, Bacterial growth curve analysis • Bioinformatics: Docking, In silico ADMET predictions, BLAST, FASTA, Multiple sequence alignment, Phylogenetic tree construction • Immunological studies: ELISA, Immunocytochemistry From past one and half year I developed bacterial detection kits for water testing. Further, developed the probiotics for aqua and agriculture farming. Presently working on the commercialization of probiotics and development of other diagnostic kits to improve the human health by reducing the antimicrobial resistance development indirectly.

Subject Matter Expertise

• ★ Biotechnology & Bioengineering
• ★ Cancer Research
• ☆ Microbiology
• ☆ Biostatistics
• ☆ Molecular Biology
• ☆ RNA Sequencing
• ☆ Recombinant Protein Expression
• ☆ Nanobiotechnology
• ☆ Microbial Biotechnology
• ☆ Agriculture
• ☆ Industrial Biotechnology
• ☆ Protein/Enzyme Engineering
• ☆ Blotting

Services

• Writing
• Research
• Data & AI
• Product Development

Writing Technical Writing, Newswriting

Research Fact Checking, Gray Literature Search, Systematic Literature Review, Secondary Data Collection

Data & AI Statistical Analysis

Product Development Formulation, Stability/Shelf Life Testing

Work Experience

Project Assistant II

Indian Institute of Chemical Technology

December 2006 - March 2012

Education

Ph.D (Medicinal chemistry and pharmacology & IPS (Institute of pharmaceutical sciences))

Indian Institute of Chemical Technology and King's college London

September 2012 - July 2019

Certifications

• Certification details not provided.

Publications

JOURNAL ARTICLE

(2017). Design, Synthesis and Biological Evaluation of 2-Anilinopyridyl-Linked Oxindole Conjugates as Potent Tubulin Polymerisation Inhibitors . ChemistrySelect.

(2017). Rational design and synthesis of 2-anilinopyridinyl-benzothiazole Schiff bases as antimitotic agents . Bioorganic & Medicinal Chemistry Letters.

(2017). Design, Synthesis, and in vitro and in vivo Evaluations of (Z )-3,4,5-Trimethoxystyrylbenzenesulfonamides/sulfonates as Highly Potent Tubulin Polymerization Inhibitors . ChemMedChem.

(2017). Design, Synthesis, and in vitro and in vivo Evaluations of (Z)-3,4,5-Trimethoxystyrylbenzenesulfonamides/sulfonates as Highly Potent Tubulin Polymerization Inhibitors . ChemMedChem.

(2017). Synthesis and biological evaluation of cis -restricted triazole/tetrazole mimics of combretastatin-benzothiazole hybrids as tubulin polymerization inhibitors and apoptosis inducers . Bioorganic & Medicinal Chemistry.

Subba Rao AV, Swapna K, Shaik SP, Lakshma Nayak V, Srinivasa Reddy T, Sunkari S, Shaik TB, Bagul C, Kamal A(2016). Synthesis and biological evaluation of cis-restricted triazole/tetrazole mimics of combretastatin-benzothiazole hybrids as tubulin polymerization inhibitors and apoptosis inducers . Bioorganic & medicinal chemistry.

(2016). Design and synthesis of cis -restricted benzimidazole and benzothiazole mimics of combretastatin A-4 as antimitotic agents with apoptosis inducing ability . Bioorganic & Medicinal Chemistry Letters.

(2016). Synthesis and Biological Evaluation of 1,2,3-triazole tethered Pyrazoline and Chalcone Derivatives . Chemical Biology & Drug Design.

(2016). Design, synthesis and antiproliferative activity of the new conjugates of E7010 and resveratrol as tubulin polymerization inhibitors . Organic & Biomolecular Chemistry.

Kamal A, Ashraf M, Basha ST, Ali Hussaini SM, Singh S, Vishnuvardhan MV, Kiran B, Sridhar B(2015). Design, synthesis and antiproliferative activity of the new conjugates of E7010 and resveratrol as tubulin polymerization inhibitors . Organic & biomolecular chemistry.

Kamal A, Shaik TB, Malik MS(2015). Embracing synthetic lethality of novel anticancer therapies . Expert opinion on drug discovery.

Kamal A, Hussaini SM, Nayak VL, Malik MS, Sucharitha ML, Shaik TB, Ashraf M, Bagul C(2014). Synthesis of 2-anilinopyridine dimers as microtubule targeting and apoptosis inducing agents . Bioorganic & medicinal chemistry.

(2014). Synthesis of 2-anilinopyridine dimers as microtubule targeting and apoptosis inducing agents . Bioorganic & Medicinal Chemistry.

(2014). Design and Synthesis of Imidazo[2,1-b]thiazole-Chalcone Conjugates: Microtubule-Destabilizing Agents . ChemMedChem.

(2014). Synthesis and biological evaluation of 1,2,3-triazole linked aminocombretastatin conjugates as mitochondrial mediated apoptosis inducers . Bioorganic & Medicinal Chemistry.

Kamal A, Balakrishna M, Nayak VL, Shaik TB, Faazil S, Nimbarte VD(2014). Design and synthesis of imidazo[2,1-b]thiazole-chalcone conjugates: microtubule-destabilizing agents . ChemMedChem.

Kamal A, Shaik B, Nayak VL, Nagaraju B, Kapure JS, Shaheer Malik M, Shaik TB, Prasad B(2014). Synthesis and biological evaluation of 1,2,3-triazole linked aminocombretastatin conjugates as mitochondrial mediated apoptosis inducers . Bioorganic & medicinal chemistry.

(2012). Synthesis of chalcone-amidobenzothiazole conjugates as antimitotic and apoptotic inducing agents . Bioorganic & Medicinal Chemistry.

(2012). Synthesis of terphenyl benzimidazoles as tubulin polymerization inhibitors . European Journal of Medicinal Chemistry.

(2012). Synthesis, Anticancer Activity and Inhibition of Tubulin Polymerization of Bisindole Sulfonates . Letters in Drug Design & Discovery.

(2012). p53–Mdm2 inhibitors: patent review (2009 – 2010) . Expert Opinion on Therapeutic Patents.

(2012). Synthesis and anticancer activity of 4β-alkylamidochalcone and 4β-cinnamido linked podophyllotoxins as apoptotic inducing agents . European Journal of Medicinal Chemistry.

(2011). Synthesis and Antitumor Evaluation of Nitrovinyl Biphenyls: Anticancer Agents Based on Allocolchicines . ChemMedChem.

(2011). Synthesis and Anticancer Activities of New Benzothiadiazinyl Hydrazinecarboxamides and Anilino[1,2,4]triazolo[1,5-b][1,2,4]thiadiazine 5,5-diones . Medicinal Chemistry.

Kamal A, Srikanth YV, Ashraf M, Khan MN, Shaik TB, Kalivendi SV, Suri N, Saxena AK(2011). Synthesis and anticancer activities of new Benzothiadiazinyl Hydrazinecarboxamides and Anilino[1,2,4]triazolo[1,5-b][1,2,4]thiadiazine 5,5-diones. Medicinal chemistry (Shariqah (United Arab Emirates)).

(2011). Synthesis, structure–activity relationship of novel substituted 4H-chromen-1,2,3,4-tetrahydropyrimidine-5-carboxylates as potential anti-mycobacterial and anticancer agents . Bioorganic & Medicinal Chemistry Letters.

(2011). Synthesis of a new 4-aza-2,3-didehydropodophyllotoxin analogues as potent cytotoxic and antimitotic agents . Bioorganic & Medicinal Chemistry.

Raju BC, Rao RN, Suman P, Yogeeswari P, Sriram D, Shaik TB, Kalivendi SV(2011). Synthesis, structure-activity relationship of novel substituted 4H-chromen-1,2,3,4-tetrahydropyrimidine-5-carboxylates as potential anti-mycobacterial and anticancer agents . Bioorganic & medicinal chemistry letters.

(2011). Synthesis and biological evaluation of 3,5-diaryl isoxazoline/isoxazole linked 2,3-dihydroquinazolinone hybrids as anticancer agents . European Journal of Medicinal Chemistry.

(2011). 2-Anilinonicotinyl linked 1,3,4-oxadiazole derivatives: Synthesis, antitumour activity and inhibition of tubulin polymerization . MedChemComm.

(2011). Bioconversion of Acrylonitrile to Acrylic Acid by Rhodococcus ruber Strain AKSH-84 . Journal of Microbiology and Biotechnology.

(2010). Two new cytotoxic labdane diterpenes from the rhizomes of Hedychium coronarium . Bioorganic & Medicinal Chemistry Letters.

(2010). Synthesis of 3,3-diindolyl oxyindoles efficiently catalysed by FeCl3 and their in vitro evaluation for anticancer activity . Bioorganic & Medicinal Chemistry Letters.

Suresh G, Reddy PP, Babu KS, Shaik TB, Kalivendi SV(2010). Two new cytotoxic labdane diterpenes from the rhizomes of Hedychium coronarium . Bioorganic & medicinal chemistry letters.

(2010). Synthesis and biological evaluation of cinnamido linked pyrrolo[2,1-c][1,4]benzodiazepines as antimitotic agents . European Journal of Medicinal Chemistry.

Kamal A, Srikanth YV, Khan MN, Shaik TB, Ashraf M(2010). Synthesis of 3,3-diindolyl oxyindoles efficiently catalysed by FeCl3 and their in vitro evaluation for anticancer activity . Bioorganic & medicinal chemistry letters.

Yadav JS, Satyanarayana K, Sreedhar P, Srihari P, Shaik TB, Kalivendi SV(2010). Total synthesis of (+/-)-elegansidiol, (+/-)-farnesiferol B, and (+/-)-farnesiferol D . Bioorganic & medicinal chemistry letters.

(2009). Remarkable enhancement in the DNA-binding ability of C2-fluoro substituted pyrrolo[2,1-c][1,4]benzodiazepines and their anticancer potential . Bioorganic & Medicinal Chemistry.