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Hire Dr. Ratnesh Kumar T.

India

Profile Summary

I am currently working as Application Scientist in Imperial Life Sciences with working position at NBFGR, Lucknow, India and my work includes Genomes and Isoform sequencing of on 3rd Generation Sequencer (PacBio RSII). I had successfully optimized and prepared the genomic and Iso-Seq Libraries for sequencing on PacBio RSII platform. Presently, engaged in sequencing, assembly and annotation of fungal and fish genomes using third generation sequencing platform and dedicated bioinformatics pipelines (HGAP, SMRTmake, FALCON). Have a good expertise of data generation and and analysis for the third generation sequencing system. Previously, successfully completed a project on "Bioprospecting of genes and allele mining for abiotic stress tolerance: Hypoxia tolerance in Indian catfish Clarias batrachus". The study involved:  Defining the hypoxic stress in Indian catfish Clarias batrachus by standardizing a set of physiological parameters including enzyme profiles.  Total RNA isolation by manual, kit based and automated methods.  Construction of forward and reverse subtracted cDNA libraries from individual tissues.  Bioinformatics analysis of Expressed Sequence Tags (EST) including vector removal, Blasting, cluster analysis and annotations using BLAST2GO and pathway analysis by KEGG and identification of repeat motif.  Identification of hypoxia relevant genes.  Identification of hypoxic stress related EST-SSR microsatellite markers and amplification of characterization polymorphic EST-SSR markers using native PAGE.  Planning, procurement of consumables, report preparation, data management and submission of EST sequences.  Identification of hypoxic stress biomarkers on catfish through amplification of mitochondrial primers from genomic DNA, construction of forward and reverse subtracted cDNA plamid libraries and analysis of differential expression profile from generated expressed sequence tags.  Quantitative Real time PCR analysis of differential expression profile of selected deferentially expressed transcripts. I have a Ph.D. in Functional Genomics and more than 6 years research experience in molecular biology lab having skills specializing in full range of research expertise in biological sciences. I have extensive working experience in functional genomics, bioinformatics, qRT-PCR and proteomic based studies. I am an expert troubleshooter, with a love of the science of genetics and molecular biology. I am very well trained in experimental designing and interpretation of data, written and verbal communication, team work and collaborations, completing projects on or before deadlines, quality assurance and attention to details.

Subject Matter Expertise

• ☆ Molecular Biology
• ☆ Biomarkers
• ☆ Genetics
• ☆ Genomics
• ☆ PCR
• ☆ Next Generation Sequencing (NGS)
• ☆ DNA Sequencing
• ☆ RNA Sequencing
• ☆ Biotechnology & Bioengineering
• ☆ Data Management
• ☆ Protein/Enzyme Engineering
• ☆ Audio Transcription

Services

• Writing
• Consulting
• Data & AI
• Product Development

Work Experience

Application Scientist

Imperial Life Sciences Private Limited

April 2014 - Present

Research Associate

National Bureau of Fish Genetic Resources

July 2011 - March 2014

Senior Research Fellow

National Bureau of Fish Genetic Resources

September 2009 - July 2011

Education

Ph. D. / Identification and Characterization of Genes Involved in Hypoxia Tolerance in Indian Catfish Clarias batrachus Brain (Fish Conservation Division)

National Bureau of Fish Genetic Resources

November 2009 - June 2014

Certifications

• Certification details not provided.

Publications

JOURNAL ARTICLE

(2016). Hypoxic stress -responsive genes in air breathing catfish, Clarias magur (Hamilton 1822) and their possible physiological adaptive function . Fish & Shellfish Immunology.

(2016). Expression profile and in silico characterization of novel RTF2h gene under oxidative stress in Indian catfish, Clarias magur (Hamilton 1822). Mol Biol Rep.

(2015). Complete mitogenome of Indian mottled eel, Anguilla bengalensis bengalensis (Gray, 1831) through PacBio RSII sequencing. MITOCHONDRIAL DNA.

(2015). Hypoxia induced altered expression of heat shock protein genes (Hsc71, Hsp90a and Hsp10) in Indian Catfish, Clarias batrachus (Linnaeus, 1758) under oxidative stress. Molecular Biology Reports.

(2014). Identification of candidate reference genes for quantitative expression analysis by real-time PCR for hypoxic stress in Indian catfish, Clarias batrachus (Linnaeus, 1758). International Aquatic Research.

(2014). Hypoxia influences expression profile of Pleckstrin homology-like domain, family A, member 2 in Indian catfish, Clarias batrachus (Linnaeus, 1758): A new candidate gene for hypoxia tolerance in fish. Journal of Biosciences.

(2013). Molecular characterization and expression analysis of PPP1R3C in hypoxia-tolerant Indian catfish, Clarias batrachus (Linnaeus, 1758) under hypoxia. Gene.

(2013). Molecular characterization and expression analysis of three hypoxia-inducible factor alpha subunits, HIF-1a, -2a and -3a in hypoxia-tolerant Indian catfish, Clarias batrachus [Linnaeus, 1758]. Molecular Biology Reports.

(2013). Physiological responses to acute experimental hypoxia in the air-breathing Indian catfish, Clarias batrachus (Linnaeus, 1758). Journal of Biosciences.

(2013). Molecular Characterization and expression analysis of a novel Cystatin-like gene in a hypoxia-tolerant Indian catfish, Clarias batrachus [Linnaeus, 1758]. Fish and Shellfish Immunology.

(2012). Characterization of 27 novel gene-associated SSR markers in Indian catfish, Clarias batrachus (Linnaeus, 1758) and their application in genetic diversity analysis. Molecular Ecology Resources.

(2012). Development of EST derived SSRs and SNPs as a genomic resource in Indian catfish, Clarias batrachus. Molecular Biology Reports.