Profile Details

Create Project

★★★★★

☆☆☆☆☆

USD 240 /hr

★★★★★

☆☆☆☆☆

Hire Dr. Jonathan M.

United States

USD 240 /hr

Protein engineer & biochemist with 20+ years experience

Profile Summary

My "bench" research is focused on protein engineering, most recently with the development of genetically encoded fluorescent biosensors. I have also done some antibody engineering. My "literature" research is focused on cancer therapeutics and diagnostics.

Subject Matter Expertise

• ☆ Biochemistry
• ☆ Structural Biology
• ☆ Protein/Enzyme Engineering
• ☆ Antibody Engineering

Services

• Writing
• Research
• Consulting
• Product Development

Work Experience

Senior Scientist

Howard Hughes Medical Inst

October 2006 - Present

Senior Scientist

ImClone Systems

June 2004 - September 2006

Postdoc

Genentech

June 2001 - June 2004

Education

PhD

Duke University

September 1995 - June 2001

BS

Cornell University

September 1991 - June 1995

Certifications

• Certification details not provided.

Publications

JOURNAL ARTICLE

Manuel Alexander Mohr, Daniel Bushey, Abhi Aggarwal, Jonathan S. Marvin, Jeong Jun Kim, Emiliano Jimenez Marquez, Yajie Liang, Ronak Patel, John J. Macklin, Chi-Yu Lee, et al.(2020). {jYCaMP}: an optimized calcium indicator for two-photon imaging at fiber laser wavelengths . Nature Methods. Springer Science and Business Media {LLC}

Paula K. Zhu, W. Sharon Zheng, Peng Zhang, Miao Jing, Philip M. Borden, Farhan Ali, Kaiming Guo, Jiesi Feng, Jonathan S. Marvin, Yali Wang, et al.(2020). Nanoscopic Visualization of Restricted Nonvolume Cholinergic and Monoaminergic Transmission with Genetically Encoded Sensors . Nano Letters. American Chemical Society ({ACS})

Thomas P. Jensen, Kaiyu Zheng, Nicholas Cole, Jonathan S. Marvin, Loren L. Looger, Dmitri A. Rusakov(2019). Multiplex imaging relates quantal glutamate release to presynaptic Ca2$\mathplus$ homeostasis at multiple synapses in situ . Nature Communications. 10. (1). Springer Science and Business Media {LLC}

Abbas Kazemipour, Ondrej Novak, Daniel Flickinger, Jonathan S. Marvin, Ahmed S. Abdelfattah, Jonathan King, Philip M. Borden, Jeong Jun Kim, Sarah H. Al-Abdullatif, Parker E. Deal, et al.(2019). Author Correction: Kilohertz frame-rate two-photon tomography . Nature Methods. 16. (9). p. 932--932. Springer Science and Business Media {LLC}

Carlos Manlio Díaz‐García, Carolina Lahmann, Juan Ramón Martínez‐François, Binsen Li, Dorothy Koveal, Nidhi Nathwani, Mahia Rahman, Jacob P. Keller, Jonathan S. Marvin, Loren L. Looger, et al.(2019). Quantitative in vivo imaging of neuronal glucose concentrations with a genetically encoded fluorescence lifetime sensor . Journal of Neuroscience Research. Wiley

Jonathan S. Marvin, Yoshiteru Shimoda, Vincent Magloire, Marco Leite, Takashi Kawashima, Thomas P. Jensen, Ilya Kolb, Erika L. Knott, Ondrej Novak, Kaspar Podgorski, et al.(2019). A genetically encoded fluorescent sensor for in vivo imaging of {GABA} . Nature Methods. 16. (8). p. 763--770. Springer Science and Business Media {LLC}

Abbas Kazemipour, Ondrej Novak, Daniel Flickinger, Jonathan S. Marvin, Ahmed S. Abdelfattah, Jonathan King, Philip M. Borden, Jeong Jun Kim, Sarah H. Al-Abdullatif, Parker E. Deal, et al.(2019). Kilohertz frame-rate two-photon tomography . Nature Methods. 16. (8). p. 778--786. Springer Science and Business Media {LLC}

Rui Liu, Zengyi Li, Jonathan S. Marvin, David Kleinfeld(2019). Direct wavefront sensing enables functional imaging of infragranular axons and spines . Nature Methods. 16. (7). p. 615--618. Springer Science and Business Media {LLC}

Jonathan S. Marvin, Benjamin Scholl, Daniel E. Wilson, Kaspar Podgorski, Abbas Kazemipour, Johannes Alexander Müller, Susanne Schoch, Francisco José Urra Quiroz, Nelson Rebola, Huan Bao, et al.(2019). Author Correction: Stability, affinity, and chromatic variants of the glutamate sensor {iGluSnFR} . Nature Methods. 16. (4). p. 351--351. Springer Science and Business Media {LLC}

Jonathan S. Marvin, Benjamin Scholl, Daniel E. Wilson, Kaspar Podgorski, Abbas Kazemipour, Johannes Alexander Müller, Susanne Schoch, Francisco José Urra Quiroz, Nelson Rebola, Huan Bao, et al.(2019). Publisher Correction: Stability, affinity, and chromatic variants of the glutamate sensor {iGluSnFR} . Nature Methods. 16. (2). p. 206--206. Springer Science and Business Media {LLC}

Jonathan S. Marvin, Benjamin Scholl, Daniel E. Wilson, Kaspar Podgorski, Abbas Kazemipour, Johannes Alexander Müller, Susanne Schoch, Francisco José Urra Quiroz, Nelson Rebola, Huan Bao, et al.(2018). Stability, affinity, and chromatic variants of the glutamate sensor {iGluSnFR} . Nature Methods. 15. (11). p. 936--939. Springer Science and Business Media {LLC}

(2016). Falling apart. Elife.

(2014). Conditions and constraints for astrocyte calcium signaling in the hippocampal mossy fiber pathway. Neuron.

(2014). Role of adaptor TrfA and ClpPC in controlling levels of SsrA-tagged proteins and antitoxins in Staphylococcus aureus. J. Bacteriol.

(2013). An optimized fluorescent probe for visualizing glutamate neurotransmission. Nat. Methods.

(2013). Two-photon imaging of nonlinear glutamate release dynamics at bipolar cell synapses in the mouse retina. J. Neurosci.

(2013). Genetically encoded calcium indicators for multi-color neural activity imaging and combination with optogenetics. Front Mol Neurosci.

(2012). Optimization of a GCaMP calcium indicator for neural activity imaging. J. Neurosci.

(2011). A genetically encoded, high-signal-to-noise maltose sensor. Proteins.

(2011). Structure of the Escherichia coli phosphonate binding protein PhnD and rationally optimized phosphonate biosensors. J. Mol. Biol.

(2009). Crystal structures of the GCaMP calcium sensor reveal the mechanism of fluorescence signal change and aid rational design. J. Biol. Chem.

(2009). Engineering human IgG1 affinity to human neonatal Fc receptor: impact of affinity improvement on pharmacokinetics in primates. J. Immunol.

(2008). Crystallization and preliminary X-ray characterization of the genetically encoded fluorescent calcium indicator protein GCaMP2. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun.

(2006). Bispecific antibodies for dual-modality cancer therapy: killing two signaling cascades with one stone. Curr Opin Drug Discov Devel.

(2005). Recombinant approaches to IgG-like bispecific antibodies. Acta Pharmacol. Sin.

(2003). Redesigning an antibody fragment for faster association with its antigen. Biochemistry.

(2001). Manipulation of ligand binding affinity by exploitation of conformational coupling. Nat. Struct. Biol.

(2001). Conversion of a maltose receptor into a zinc biosensor by computational design. Proc. Natl. Acad. Sci. U.S.A.

(1998). Protein engineering and the development of generic biosensors. Trends Biotechnol.

(1997). The rational design of allosteric interactions in a monomeric protein and its applications to the construction of biosensors. Proc. Natl. Acad. Sci. U.S.A.

OTHER

Philip M. Borden, Peng Zhang, Amol V. Shivange, Jonathan S. Marvin, Joseph Cichon, Chuntao Dan, Kaspar Podgorski, Antonio Figueiredo, Ondrej Novak, Masashi Tanimoto, et al.(2020). A fast genetically encoded fluorescent sensor for faithful in vivo acetylcholine detection in mice, fish, worms and flies . Cold Spring Harbor Laboratory

Manuel Alexander Mohr, Daniel Bushey, Abhi Aggarwal, Jonathan S. Marvin, Emiliano Jimenez Marquez, Yajie Liang, Ronak Patel, John J. Macklin, Chi-Yu Lee, Douglas S. Kim, et al.(2019). {jYCaMP}: An optimized calcium indicator for two-photon imaging at fiber laser wavelengths . Cold Spring Harbor Laboratory

Jacob P. Keller, Jonathan S. Marvin, Haluk Lacin, William C. Lemon, Jamien Shea, Soomin Kim, Richard T. Lee, Minoru Koyama, Philipp J. Keller, Loren L. Looger(2019). In vivo glucose imaging in multiple model organisms with an engineered single-wavelength sensor . Cold Spring Harbor Laboratory