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Profile Details
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USD 250 /hr
Hire Dr. John LaCava
United States
USD 250 /hr

Molecular life scientist with decades of experience in successfully executing experimental R&D projects

Profile Summary
Subject Matter Expertise
Services
Work Experience

Principal Investigator, Associate Professor

University Medical Center Groningen

July 2019 - Present

Research Associate Professor

Rockefeller University

June 2019 - Present

Education

PhD Molecular Genetics

University of Edinburgh

September 2000 - October 2005

Certifications
  • Certification details not provided.
Publications
JOURNAL ARTICLE
John LaCava, Yuhui Dou, Isabelle Barbosa, Hua Jiang, Claudia Iasillo, Kelly R Molloy, Wiebke Manuela Schulze, Stephen Cusack, Manfred Schmid, Hervé Le Hir, et al.(2020). {NCBP}3 positively impacts {mRNA} biogenesis . Nucleic Acids Research. 48. (18). p. 10413--10427. Oxford University Press ({OUP})
(2020). Ncbp3 Positively Impacts Mrna Biogenesis . Nucleic Acids Research.
(2019). To the Editor in Reply to Dr. Crow's Letter . Arthritis & Rheumatology.
John LaCava, Tomas Mustelin, Kennedy Ukadike, Marty Taylor(2019). Reply . Arthritis {\&} Rheumatology. 72. (2). p. 376--377. Wiley
John LaCava, Victoria Carter, Martin S. Taylor, Shu Ying Liang, Cecilia Mustelin, Kennedy C. Ukadike, Anders Bengtsson, Christian Lood, Tomas Mustelin(2019). High Prevalence and Disease Correlation of Autoantibodies Against p40 Encoded by Long Interspersed Nuclear Elements in Systemic Lupus Erythematosus . Arthritis {\&} Rheumatology. 72. (1). p. 89--99. Wiley
John LaCava, Elodie Pronier, Paolo Cifani, Tiffany R. Merlinsky, Katharine Barr Berman, Amritha Varshini Hanasoge Somasundara, Raajit K. Rampal, Karen E. Wei, Friederike Pastore, Jesper L.V. Maag, et al. (2018). Targeting the CALR interactome in myeloproliferative neoplasms . JCI Insight.
John LaCava, Paolo Mita, Aleksandra Wudzinska, Xiaoji Sun, Joshua Andrade, Shruti Nayak, David J Kahler, Sana Badri, Beatrix Ueberheide, Chi Y Yun, et al.(2018). {LINE}-1 protein localization and functional dynamics during the cell cycle . {eLife}. 7. {eLife} Sciences Organisation, Ltd.
Winczura K, Schmid M, Iasillo C, Molloy KR, Harder LM, Andersen JS, LaCava J, Jensen TH(2018). Characterizing ZC3H18, a Multi-domain Protein at the Interface of RNA Production and Destruction Decisions . Cell reports.
Taylor MS, Altukhov I, Molloy KR, Mita P, Jiang H, Adney EM, Wudzinska A, Badri S, Ischenko D, Eng G, et al.(2018). Dissection of affinity captured LINE-1 macromolecular complexes . eLife.
John LaCava, Yinxing Ma, Licia Silveri, Svetlana Dokudovskaya(2017). Tumor suppressor {NPRL}2 induces {ROS} production and {DNA} damage response . Scientific Reports. 7. (1). Springer Nature
John LaCava, Alexandra Santanach, Enrique Blanco, Hua Jiang, Kelly R. Molloy, Miriam Sansó, Lluis Morey, Luciano Di Croce(2017). The Polycomb group protein {CBX}6 is an essential regulator of embryonic stem cell identity . Nature Communications. 8. (1). Springer Nature
John LaCava, Michal Domanski(2017). Affinity Purification of the {RNA} Degradation Complex, the Exosome, from {HEK}-293 Cells . {BIO}-{PROTOCOL}. 7. (8). Bio-Protocol, {LLC}
John LaCava, Michal Domanski(2017). {RNA} Degradation Assay Using {RNA} Exosome Complexes, Affinity-purified from {HEK}-293 Cells . {BIO}-{PROTOCOL}. 7. (8). Bio-Protocol, {LLC}
(2016). Protein Complex Affinity Capture from Cryomilled Mammalian Cells . Journal of visualized experiments : JoVE.
John LaCava, Michal Domanski, Paula Upla, William J. Rice, Kelly R. Molloy, Natalia E. Ketaren, David L. Stokes, Torben Heick Jensen, Michael P. Rout(2016). Purification and analysis of endogenous human {RNA} exosome complexes . {RNA}. 22. (9). p. 1467--1475. Cold Spring Harbor Laboratory
John LaCava, Javier Fernandez-Martinez, Michael P. Rout(2016). Density Gradient Ultracentrifugation to Isolate Endogenous Protein Complexes after Affinity Capture . Cold Spring Harbor Protocols. 2016. (7). p. pdb.prot087957. Cold Spring Harbor Laboratory
John LaCava, Javier Fernandez-Martinez, Zhanna Hakhverdyan, Michael P. Rout(2016). Optimized Affinity Capture of Yeast Protein Complexes . Cold Spring Harbor Protocols. 2016. (7). p. pdb.prot087932. Cold Spring Harbor Laboratory
John LaCava, Javier Fernandez-Martinez, Michael P. Rout(2016). Native Elution of Yeast Protein Complexes Obtained by Affinity Capture . Cold Spring Harbor Protocols. 2016. (7). p. pdb.prot087940. Cold Spring Harbor Laboratory
John LaCava, Javier Fernandez-Martinez, Zhanna Hakhverdyan, Michael P. Rout(2016). Protein Complex Purification by Affinity Capture . Cold Spring Harbor Protocols. 2016. (7). p. pdb.top077545. Cold Spring Harbor Laboratory
(2016). Characterization of L1-Ribonucleoprotein Particles . Methods in molecular biology.
(2015). Interactomic and Enzymatic Analyses of Distinct Affinity Isolated Human Retrotransposon Intermediates. Protein science.
John LaCava, Lixin Dai, Martin S Taylor, Jef D Boeke(2014). Expression and detection of {LINE}-1 {ORF}-encoded proteins . Mobile Genetic Elements. 4. (3). p. e29319. Informa {UK} Limited
LaCava, John, Chandramouli, Nagarajan, Jiang, Hua, Rout, Michael P.(2013). Improved Native Isolation of Endogenous Protein A-Tagged Protein Complexes . Biotechniques. 54. (4). p. 213-216.
Andersen, P.R., Domanski, M., Kristiansen, M.S., Storvall, H., Ntini, E., Verheggen, C., Schein, A., Bunkenborg, J., Poser, I., Hallais, M., et al.(2013). The human cap-binding complex is functionally connected to the nuclear RNA exosome . Nature Structural and Molecular Biology. 20. (12). p. 1367-1376.
Taylor, M.S., Lacava, J., Mita, P., Molloy, K.R., Huang, C.R.L., Li, D., Adney, E.M., Jiang, H., Burns, K.H., Chait, B.T., et al.(2013). Affinity proteomics reveals human host factors implicated in discrete stages of LINE-1 retrotransposition . Cell. 155. (5). p. 1034-1048.
(2012). High-Throughput, Single-Step Purification of Affinity-Tagged Protein Complexes. FASEB journal.
LaCava, John, Chait, Brian T, Domanski, Michal, Jensen, Torben Heick, Jiang, Hua, Molloy, Kelly, Rout, Michael P(2012). Improved methodology for the affinity isolation of human protein complexes expressed at near endogenous levels . BioTechniques. 0. (0). p. 1-6.
LaCava, John, Chait, Brian T., Domanski, Michal, Fenyo, David, Hakhverdyan, Zhanna, Hough, Loren, Jensen, Torben Heick, Oroskar, Anil, Oroskar, Asha, Rout, Michael P.(2010). High-Throughput, Single-Step Purification of Affinity-Tagged Protein Complexes. FASEB Journal. 24.
(2006). RNA-quality control by the exosome . Nature reviews. Molecular cell biology.
LaCava, John, Houseley, Jonathan, Tollervey, David(2006). RNA-quality control by the exosome . Nature Reviews Molecular Cell Biology. 7. (7). p. 529-539.
Houseley, J, Jacquier, A, LaCava, J, Petfalski, E, Saveanu, C, Thompson, E, Tollervey, D(2005). RNA degradation by the exosome is promoted by a nuclear polyade nylation complex . Cell. 121. (5). p. 713-724.
OTHER
John LaCava, Yuhui Dou, Isabelle Barbosa, Hua Jiang, Claudia Iasillo, Kelly R. Molloy, Wiebke Manuela Schulze, Stephen Cusack, Manfred Schmid, Hervé Le Hir, et al.(2020). {NCBP}3 is a productive {mRNP} component . Cold Spring Harbor Laboratory
John LaCava, Mehrnoosh Oghbaie, Petr Šulc, David Fenyö, Michael Pennock(2019). Complex$\mathplus$: Aided Decision-Making for the Study of Protein Complexes . Cold Spring Harbor Laboratory