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USD 20 /hr

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Hire Dr. João N.

Netherlands

USD 20 /hr

CRISPR & compound screening expert | Pre-clinical (in vitro and in vivo) drug validation expert

Profile Summary

For the past 5 years I have worked in the lab of Prof. Rene Bernards at the Netherlands Cancer Institute as a PhD student. I am now close to my graduation date and I want to put some of the skills I have mastered to use, as a consultant. Throughout these years I have performed and validated over 20 genetic (CRISPR) and compound screens. This experience lead to the discovery and development of novel method that increases the success rate of target identification via CRISPR screening (manuscript submited) -therefore I consider this to be my main expertise. Additionally, I have also lead the pre-clinical (in vitro and in vivo) validation of 2 different novel drug combinations, one of which is currently being taken forward into a clinical trial. Finally, throughout this process I have also writen several scientific papers (at the moment 2 are accepted, 1 is under review and 3 are close to submission) and therefore I can also provide advice in scientific writing.

Subject Matter Expertise

• ★ Cell Signaling Pathways
• ★ Gene Editing/CRISPR
• ★ Cancer Research
• ☆ STEM
• ☆ Molecular Biology
• ☆ Cell Culture
• ☆ Drug Development
• ☆ Small Molecule Drug Development
• ☆ Preclinical Research
• ☆ Research
• ☆ Technical & Scientific Writing
• ☆ Science Communication

Services

• Writing
• Research
• Consulting
• Data & AI
• Product Development

Writing Clinical Trial Documentation, Technical Writing, Newswriting

Research Feasibility Study, Fact Checking, Gray Literature Search, Systematic Literature Review, Secondary Data Collection

Consulting Scientific and Technical Consulting

Data & AI Statistical Analysis, Data Visualization

Product Development Formulation

Work Experience

PhD student

Nederlands Kanker Instituut - Antoni van Leeuwenhoek Ziekenhuis

September 2015 - Present

Education

Master of Science (Chemistry Department)

Universidade de Aveiro

September 2013 - July 2015

Bachelor of Science (Chemistry Department)

Universidade de Aveiro

September 2010 - June 2014

Certifications

• Certification details not provided.

Publications

JOURNAL ARTICLE

João M. Fernandes Neto, Ernest Nadal, Evert Bosdriesz, Salo N. Ooft, Lourdes Farre, Chelsea McLean, Sjoerd Klarenbeek, Anouk Jurgens, Hannes Hagen, Liqin Wang, et al. (2020). Multiple low dose therapy as an effective strategy to treat EGFR inhibitor-resistant NSCLC tumours . Nature Communications.

Wang, L., Leite de Oliveira, R., Wang, C., Fernandes Neto, J.M., Mainardi, S., Evers, B., Lieftink, C., Morris, B., Jochems, F., Willemsen, L., et al.(2017). High-Throughput Functional Genetic and Compound Screens Identify Targets for Senescence Induction in Cancer . Cell Reports. 21. (3). p. 773-783.

OTHER

João M. Fernandes Neto, Ernest Nadal, Salo N Ooft, Evert Bosdriesz, Lourdes Farre, Chelsea McLean, Sjoerd Klarenbeek, Anouk Jurgens, Hannes Hagen, Liqin Wang, et al.(2019). Multiple Low Dose ({MLD}) therapy: an effective strategy to treat {EGFR} inhibitor-resistant {NSCLC} tumours . Cold Spring Harbor Laboratory