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Hire Dr. Yakov V.

Ukraine

USD 50 /hr

PhD in Molecular Biology (2007; Univ IL Chicago). Expertise: NGS, variant analysis for WES, molcell biology

Profile Summary

EDUCATION: 2000 Lomonosov Moscow State University, Moscow, Russia B.S., Biological Sciences specializing in biophysics 2007 University of Illinois at Chicago, Chicago, IL. PhD, Molecular Biology RESEARCH AND WORKING EXPERIENCE: 2000-2007 University of Illinois at Chicago, Department of Biological Sciences, Research assistant / Phd student. PI: Dr. Susan Liebman Thesis: Mutational analysis of the yeast prion proteins Sup35 and Rnq1. 2005-2009 Ingenuity Systems, Inc (part-time). Corresponding scientist. Building and editing a knowledge database of molecular interactions in biological systems. 2010-2012 Institut de génétique et de biologie moléculaire et cellulaire, Illkirch, France, Postdoctoral fellow. PI: Dr. Jean-Marc Egly. Projects : 1. Transactivation properties of PPARγ as a function of its phosphorylation status 2. Genotyping Mediator components using next-generation sequencing 2013-2016 Cell Therapy Center Emcell, Kyiv, Ukraine. Head of the molecular diagnositcs unit. 1. Expression assays for pluripotence markers in stem-cells suspensions (qRT-PCR, RNAseq) 2. Screening and identification of viral and microbial pathogens in stem-cell suspensions 2016-2017 Research and Control Institute of Biotechnology and Strains of Microorganisms, Kyiv, Ukraine. Research associate. Molecular genotyping of the State Collection of Microorganisms 2017-2020 IMG, llumina Channel Parnter, Kyiv, Ukraine. Senior application scientist Support and customization of Illumina-based NGS solutions PUBLICATIONS: Yakov Vitrenko, Elena Gracheva, Janet Richmond and Susan Liebman. Visualization of aggregation of the Rnq1 prion domain and cross-seeding interactions with Sup35NM. Journal of Biological Chemistry, 2007 Jan 19;282(3):1779-87 Yakov Vitrenko, Mariana Pavon, Stephen Stone and Susan Liebman. Propagation of the [PIN+] prion by Rnq1 fragments. Current Genetics 2007 May;51(5):309-19 Yakov Vitrenko, Iryna Kostenko, Kateryna Kulebyakina, Alla Duda, Maria Klunnik, Khrystyna Sorochynska. Fetal tissues tested for microbial sterility by culture- and PCR-based methods can be safely used in clinics. Cell Transplantation 2017 Feb 16;26(2):339-350 Yakov Vitrenko, Iryna Kostenko, Kateryna Kulebyakina and Khrystyna Sorochynska. Prevalence, mother-to-fetus transmission and sequence variability of the E2 glycoprotein of pegivirus estimated from screening donors of stem-cell containing material. Virol J. 2017 Aug 31;14(1):167 Yakov Vitrenko and Oleg Deriabin. Dual-target strategy for detection of Chlamydia trachomatis by real-time PCR featuring an innovative internal control system. Biopolymers and Cell, 2018;34(2):117-126 GRANTS AND FELLOWSHIPS: 2003, 2006 Graduate student research assistantship, University of Illinois at Chicago 2009 The joint grant of the Ministy of Healthcare of Ukraine and DNA Lab, Ltd for designing PCR-based methodology for the detection and genotyping of human papillamovirus. 2010 Individual postdoctoral fellowship, INSERM, France 2015 Research fellowship of the Ministry of Defense of Ukraine for the molecular genotyping of the Collection of Microorganisms PROFESSIONAL SKILLS: 1. Molecular cloning, PCR and other basic DNA/RNA manipulations 2. Next-generation sequencing: from library preparation (Illumina Nextera) to data analysis 3. Sequence analysis (alignment, phylogenetic analysis, evolutionary models) 4. Recombinant protein purification 5. Amyloid and prion proteins 6. Protein and chromatin immunoprecipitation 7. In vitro transcription 8. Yeast genetics: screens, libraries, gene manipulations 9. Mammalian cells: gene expression assays, transfection, lentiviral transduction, reporter assays, embryonic stem cell differentiation INSTRUCTIONAL EXPERIENCE: 2000-2002, 2004-2005, Teaching Assistant, Genetics , levels 1 and 2, University of Illinois at Chicago, Department of Biological Sciences 2003 Scientific Reasoning Tutor, University of Illinois at Chicago, Early outreach program 2012-2013 Supervising master students Institut de génétique et de biologie moléculaire et cellulaire, Illkirch, France 2014 – 2016 Training lab and research associates Emcell, The Cell therapy center, Kyiv, Ukraine 2017-2018 NGS training sessions (lab procedures and data analysis) Illumina users in Ukraine LANGUAGES SPOKEN: Ukrainian, English, French, Russian (all fluent)

Subject Matter Expertise

• ★ Next Generation Sequencing (NGS)
• ☆ Molecular Biology
• ☆ Genetics
• ☆ PCR
• ☆ DNA Sequencing
• ☆ Recombinant DNA
• ☆ Cell Biology
• ☆ Recombinant Protein Expression
• ☆ Bioinformatics
• ☆ Clinical Laboratory Science
• ☆ Medical Writing
• ☆ Technical & Scientific Writing
• ☆ Grant Writing

Services

• Writing
• Research
• Consulting
• Data & AI
• Product Development

Writing Clinical Trial Documentation, Technical Writing, Copywriting, Newswriting

Research Market Research, Feasibility Study, Fact Checking, Gray Literature Search, Systematic Literature Review, Secondary Data Collection

Consulting Scientific and Technical Consulting

Data & AI Statistical Analysis

Product Development Formulation

Work Experience

Scientific consultant

IMG

April 2017 - Present

Brand Manager / Scientific consultant

IMG - Illumina Channel Partner

March 2017 - Present

Education

PhD Molecular Biology

University of Illinois at Chicago (UIC)

July 2000 - May 2007

Bachelor of sciences (Biology)

Lomonosov Moscow State University

September 1995 - May 2000

Certifications

• Certification details not provided.

Publications

JOURNAL ARTICLE

Dmytro Sirokha and Olexandra Gorodna and Yakov Vitrenko and Nataliya Zelinska and Rafal Ploski and Serge Nef and Jadwiga Jaruzelska and Kamila Kusz-Zamelczyk and Ludmila Livshits(2021). A Novel WT1 Mutation Identified in a 46,XX Testicular/Ovotesticular DSD Patient Results in the Retention of Intron 9 . MDPI Biology. 10. (12). p. 1248. {MDPI} {AG}

A dual-target strategy for the detection of Chlamydia trachomatis by real-time PCR / Y.A. Vitrenko, O.M. Deryabin // Вiopolymers and Cell .

(2017). Prevalence of human pegivirus-1 and sequence variability of its E2 glycoprotein estimated from screening donors of fetal stem cell-containing material . Virology journal.

(2016). Fetal Tissues Tested for Microbial Sterility by Culture- and PCR-Based Methods Can be Safely Used in Clinics . Cell transplantation.

(2007). Propagation of the [PIN+] prion by fragments of Rnq1 fused to GFP . Current genetics.

(2006). Visualization of aggregation of the Rnq1 prion domain and cross-seeding interactions with Sup35NM . The Journal of biological chemistry.