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Hire Dr. Fernando A.

United States

Profile Summary

Education: microMBA 2016 Rady School of management (UCSD). PhD in Molecular Biology & Biotechnology 2005-2010 Spanish National Research Council (CSIC), University of Murcia, Spain. PharmD 1999-2004 University of Granada, Spain. List of Publications: https://scholar.google.com/citations?user=Ue5UTOsAAAAJ Scientific Skills/Techniques: Molecular Biology: Fully competent in molecular biology techniques: RNA and DNA extraction, Southern blot, Northern blot, random and site-directed mutagenesis, single-cell RT-PCR, qPCR, 5’RACE and DNA cloning (classical, USER, Gateway, Gibson). Protein Biochemistry: Outstanding skills in protein engineering, expression and purification from bacterial, mammalian and yeast systems. Experienced with biochemical assays such as phosphatase activity, acetylcholinesterase activity and papain digestion of antibodies. Protein and antibody Purification (Affinity, IEX, SEC) via FPLC. Experience in direct ELISA, sandwich and competitive ELISA. Basic knowledge on mass spectrometry (over protein samples after tandem affinity purification). Protein Crystallography: Cutting-edge knowledge in the state of the art crystallographic facilities of Scripps (Ian Wilson collaboration). Cell Biology: Experienced in cell culture of mammalian cells, bacteria and yeast. Flow cytometry of up to 4 colors using Novocyte and BD LSRII with yeast and splenocyte samples. In vivo protein-protein interaction assays (yeast two hybrid and Bimolecular Fluorescence Complementation –BiFC- using confocal microscope). In vivo protein-DNA interaction (yeast one hybrid). Bioinformatics: Expert using Vector NTI, GraphPad and online databases. Presented data in scientific meetings. X-ray data analysis with Phenix and Coot, and protein structure analysis with Pymol software. Biophysical assays: Protein-ligand interactions through ThermoFluor and antibody-antigen interaction using Surface Plasmon Resonance (Octet). f.alemanguillen at gmail.com

Subject Matter Expertise

• ☆ Separation & Purification
• ☆ Crystallography
• ☆ Mass Spectrometry
• ☆ Plant Breeding & Genetics
• ☆ Plant Physiology
• ☆ Microbiology
• ☆ Virology
• ☆ Biochemistry
• ☆ Immunoassay
• ☆ Biochemical Assays
• ☆ Molecular Biology
• ☆ PCR
• ☆ RNA Sequencing
• ☆ Biophysics
• ☆ Microscopy
• ☆ Microbial Fermentation
• ☆ Protein/Enzyme Engineering
• ☆ Antibody Engineering
• ☆ Blotting
• ☆ Cytometry
• ☆ Spanish
• ☆ Pharmacy

Services

• Writing
• Consulting
• Data & AI
• Product Development

Work Experience

Co-founder, president and CSO

Navega Therapeutics

2018 - Present

Consultant

Inova Diagnostics

May 2018 - December 2018

Research Associate

Scripps Research Institute

October 2014 - February 2018

Postdoctoral Fellow

University of California San Diego

2010 - September 2014

Education

Ph.D. (CEBAS-CSIC)

Universidad de Murcia - Campus de Espinardo

2005 - 2009

Pharm. D

Universidad de Granada Facultad de Farmacia

1999 - 2004

Certifications

• Certification details not provided.

Publications

JOURNAL ARTICLE

Fernando Aleman, Andrea Vega-Loza, Christina Van, Ana M Moreno(2020). Gene therapies to reduce chronic pain: are we there yet? . Pain Management. Future Medicine Ltd

Ana M. Moreno, Nathan Palmer, Fernando Alemán, Genghao Chen, Andrew Pla, Ning Jiang, Wei Leong Chew, Mansun Law, Prashant Mali(2019). Author Correction: Immune-orthogonal orthologues of {AAV} capsids and of Cas9 circumvent the immune response to the administration of gene therapy . Nature Biomedical Engineering. 3. (10). p. 842--842. Springer Science and Business Media {LLC}

(2019). Immune-orthogonal orthologues of AAV capsids and of Cas9 circumvent the immune response to the administration of gene therapy . Nature Biomedical Engineering.

Fernando Aleman, Netanel Tzarum, Leopold Kong, Kenna Nagy, Jiang Zhu, Ian A. Wilson, Mansun Law(2018). Immunogenetic and structural analysis of a class of {HCV} broadly neutralizing antibodies and their precursors . Proceedings of the National Academy of Sciences. 115. (29). p. 7569--7574. Proceedings of the National Academy of Sciences

Aleman, F.(2017). The Necessity of Diploid Genome Sequencing to Unravel the Genetic Component of Complex Phenotypes . Frontiers in Genetics. 8. p. 3.

Aleman, Fernando, Yazaki, Junshi, Galli, Mary, Kim, Alice Y., Nito, Kazumasa, Chang, Katherine N., Carvunis, Anne-Ruxandra, Quan, Rosa, Nguyen, Hien, Song, Liang, et al.(2016). Mapping transcription factor interactome networks using HaloTag protein arrays . Proceedings of the National Academy of Sciences of the United States of America. 113. (29). p. E4238-47.

Aleman, Fernando, Yazaki, Junshi, Lee, Melissa, Takahashi, Yohei, Kim, Alice Y., Li, Zixing, Kinoshita, Toshinori, Ecker, Joseph R., Schroeder, Julian I.(2016). An ABA-increased interaction of the PYL6 ABA receptor with MYC2 Transcription Factor: A putative link of ABA and JA signaling . Scientific Reports. 6.

Brandt B, Munemasa S, Wang C, Nguyen D, Yong T, Yang PG, Poretsky E, Belknap TF, Waadt R, Alemán F, Schroeder JI, eLife, 2015, vol. 4 .

Brandt, B., Munemasa, S., Wang, C., Nguyen, D., Yong, T. M., Yang, P. G., Poretsky, E., Belknap, T. F., Waadt, R., Aleman, F., et al.(2015). Calcium specificity signaling mechanisms in abscisic acid signal transduction in Arabidopsis guard cells . Elife. 4. p. 25.

Rubio F, Fon M, Ródenas R, Nieves-Cordones M, Alemán F, Rivero RM, Martínez V, Physiologia plantarum, 2014, vol. 152, no. 3, pp. 558-570 .

Aleman, Fernando, Nieves-Cordones, Manuel, Martinez, Vicente, Rubio, Francisco(2014). K(+) uptake in plant roots. The systems involved, their regulation and parallels in other organisms . Journal of plant physiology. 171. (9). p. 688-95.

Alemán F, Caballero F, Ródenas R, Rivero RM, Martínez V, Rubio F, Frontiers in plant science, 2014, vol. 5, pp. 430 .

Aleman, F., Nieves-Cordones, M., Martinez, V., Rubio, F.(2011). Root K(+) Acquisition in Plants: The Arabidopsis thaliana Model . Plant and Cell Physiology. 52. (9). p. 1603-1612.

Rubio, F., Aleman, F., Nieves-Cordones, M., Martinez, V.(2010). Studies on Arabidopsis athak5, atakt1 double mutants disclose the range of concentrations at which AtHAK5, AtAKT1 and unknown systems mediate K plus uptake . Physiologia Plantarum. 139. (2). p. 220-228.

Nieves-Cordones, M., Aleman, F., Martinez, V., Rubio, F.(2010). The Arabidopsis thaliana HAK5 K(+) Transporter Is Required for Plant Growth and K(+) Acquisition from Low K(+) Solutions under Saline Conditions . Molecular Plant. 3. (2). p. 326-333.

Rubio, F., Aleman, F., Nieves-Cordones, M., Martinez, V.(2009). Differential regulation of the genes encoding the high-affinity K(+) transporters HAK5 of Thellungiella halophila and Arabidopsis thaliana in response to salinity . Comparative Biochemistry and Physiology a-Molecular & Integrative Physiology. 153A. (2). p. S188.

Aleman, F., Nieves-Cordones, M., Martinez, V., Rubio, F.(2009). Potassium/sodium steady-state homeostasis in Thellungiella halophila and Arabidopsis thaliana under long-term salinity conditions . Plant Science. 176. (6). p. 768-774.

Aleman, F., Nieves-Cordones, M., Martinez, V., Rubio, F.(2009). Differential regulation of the HAK5 genes encoding the high-affinity K(+) transporters of Thellungiella halophila and Arabidopsis thaliana . Environmental and Experimental Botany. 65. (2-3). p. 263-269.

Rubio, F., Nieves-Cordones, M., Aleman, F., Martinez, V.(2008). Relative contribution of AtHAK5 and AtAKT1 to K(+) uptake in the high-affinity range of concentrations . Physiologia Plantarum. 134. (4). p. 598-608.

Nieves-Cordones, M., Miller, A. J., Aleman, F., Martinez, V., Rubio, F.(2008). A putative role for the plasma membrane potential in the control of the expression of the gene encoding the tomato high-affinity potassium transporter HAK5 . Plant Molecular Biology. 68. (6). p. 521-532.

PREPRINT

(2019). Long-lasting Analgesia via Targetedin {vivoEpigenetic} Repression of Nav1.7 . Cold Spring Harbor Laboratory

OTHER

Ana M. Moreno, Glaucilene F. Catroli, Fernando Alemán, Andrew Pla, Sarah A. Woller, Michael Hu, Tony Yaksh, Prashant Mali(2019). Long-lasting Analgesia via Targetedin {vivoEpigenetic} Repression of Nav1.7 . Cold Spring Harbor Laboratory

Fernando Aleman, Ana M Moreno, Nathan Palmer, Genghao Chen, Andrew Pla, Wei Leong Chew, Mansun Law, Prashant Mali(2018). Exploring protein orthogonality in immune space: a case study with {AAV} and Cas9 orthologs . Cold Spring Harbor Laboratory

Fernando Aleman, Erick Giang, Mansun Law(2018). Probing the Antigenicity of {HCV} Envelope Glycoproteins by Phage Display Antibody Technology . 381--393Springer New York

DATA SET

Fernando Aleman, Julian Schroeder(2017). Faculty of 1000 evaluation for Acetate-mediated novel survival strategy against drought in plants . Faculty of 1000, Ltd.