{"id":9141,"date":"2021-05-20T04:22:28","date_gmt":"2021-05-20T04:22:28","guid":{"rendered":"https:\/\/www.kolabtree.com\/blog\/?p=9141"},"modified":"2021-05-20T04:40:52","modified_gmt":"2021-05-20T04:40:52","slug":"cristina-cosi-expression-ofcfos-mrna-is-an-indirect-marker-of-neuronal-activity","status":"publish","type":"post","link":"https:\/\/www.kolabtree.com\/blog\/cristina-cosi-expression-ofcfos-mrna-is-an-indirect-marker-of-neuronal-activity\/","title":{"rendered":"Cristina Cosi: Expression of c-fos mRNA is an indirect marker of neuronal activity"},"content":{"rendered":"

The expression of the \u201cimmediate early gene\u201d c-<\/span>fos<\/span><\/i> is an indirect marker of neuronal activity because c-fos is often expressed when neurons fire action potentials. c-<\/span>fos<\/span><\/i> mRNA expression is correlated to glutamatergic neurotransmission and has been studied extensively in psychopharmacology. RNAscope (ACD Bio) is a patented in situ mRNA detection technology (1) that uses branched DNA amplification and z paired probes\u00a0 to deliver a robust and specific assay, designed primarily for use on formalin fixed paraffin sections. The technology, adapted to frozen brain tissue sections, allowed the study of the modulation of c-<\/span>fos<\/span><\/i> expression in different neurocircuitries by a preferential D3 antagonist, potential antipsychotic, in mice treated with the NMDA glutamate receptor channel blocker MK-801 (2). The method and its validation are described in Cosi et al.,<\/span>https:\/\/doi.org\/10.1016\/j.mex.2021.101348<\/span><\/a><\/p>\n

[1] F. Wang, J. Flanagan, N. Su, L.-C. Wang, S. Bui, A. Nielson, X. Wu, H.-T. Vo, X.-J. Ma, Y. Luo, RNAscope\u00ae: A novel in situ RNA analysis platform for formalin-fixed paraffin-embedded tissues, J. Mol. Diagn. 14 (2012) 22\u201329, doi:<\/span>10.1016\/j.jmoldx.2011.08.002.<\/span><\/p>\n

[2] C. Cosi, J.C. Martel, A.L. Auclair, G. Collo, L. Cavalleri, P. Heusler, L. Leriche, F. Gaudoux, P. Sokoloff, P.C. Moser, S. GattiMcArthur, Pharmacology Profile of F17464, a Dopamine D<\/span>3 <\/span>Receptor preferential Antagonist, Eur. J. Pharmacol. 890 (2021) 173635 <\/span>https:\/\/doi.org\/10.1016\/j.ejphar.2020.173635.<\/span><\/p>\n

About the author<\/strong><\/p>\n

\"\"<\/a><\/p>\n

Cristina Cosi<\/a>, MSc, PhD, biologist and pharmacologist,\u00a0 neuroscientist by training, is freelance scientific consultant at Kolabtree.\u00a0<\/span><\/p>\n

She has dedicated her career to the understanding of the mechanisms of neuronal plasticity and to the discovery of drugs that could be beneficial against neurological (included Parkinson) and neuropsychiatric diseases and pain.<\/span><\/p><\/blockquote>\n","protected":false},"excerpt":{"rendered":"

The expression of the \u201cimmediate early gene\u201d c-fos is an indirect marker of neuronal activity because c-fos is often expressed when neurons fire action potentials. c-fos mRNA expression is correlated to glutamatergic neurotransmission and has been studied extensively in psychopharmacology. RNAscope (ACD Bio) is a patented in situ mRNA detection technology (1) that uses branched<\/p>\n

Read More<\/a><\/div>\n","protected":false},"author":12,"featured_media":9147,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[662],"tags":[],"acf":[],"yoast_head":"\nCristina Cosi: Expression of c-fos mRNA is an indirect marker of neuronal activity<\/title>\n<meta name=\"description\" content=\"Neuroscientist Cristina Cosi's research publication on the expression of c-fos mRNA as an indirect marker of neuronal activity.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.kolabtree.com\/blog\/cristina-cosi-expression-ofcfos-mrna-is-an-indirect-marker-of-neuronal-activity\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Cristina Cosi: Expression of c-fos mRNA is an indirect marker of neuronal activity\" \/>\n<meta property=\"og:description\" content=\"Neuroscientist Cristina Cosi's research publication on the expression of c-fos mRNA as an indirect marker of neuronal activity.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.kolabtree.com\/blog\/cristina-cosi-expression-ofcfos-mrna-is-an-indirect-marker-of-neuronal-activity\/\" \/>\n<meta property=\"og:site_name\" content=\"The Kolabtree Blog\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/kolabtree\" \/>\n<meta property=\"article:published_time\" content=\"2021-05-20T04:22:28+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2021-05-20T04:40:52+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.kolabtree.com\/blog\/wp-content\/uploads\/2021\/05\/Blog-headertemplate-1078x516-1.png\" \/>\n\t<meta property=\"og:image:width\" content=\"1078\" \/>\n\t<meta property=\"og:image:height\" content=\"516\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/png\" \/>\n<meta name=\"author\" content=\"Ramya Sriram\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:creator\" content=\"@kolabtree\" \/>\n<meta name=\"twitter:site\" content=\"@kolabtree\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Ramya Sriram\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"2 minutes\" \/>\n<!-- \/ Yoast SEO Premium plugin. -->","yoast_head_json":{"title":"Cristina Cosi: Expression of c-fos mRNA is an indirect marker of neuronal activity","description":"Neuroscientist Cristina Cosi's research publication on the expression of c-fos mRNA as an indirect marker of neuronal activity.","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.kolabtree.com\/blog\/cristina-cosi-expression-ofcfos-mrna-is-an-indirect-marker-of-neuronal-activity\/","og_locale":"en_US","og_type":"article","og_title":"Cristina Cosi: Expression of c-fos mRNA is an indirect marker of neuronal activity","og_description":"Neuroscientist Cristina Cosi's research publication on the expression of c-fos mRNA as an indirect marker of neuronal activity.","og_url":"https:\/\/www.kolabtree.com\/blog\/cristina-cosi-expression-ofcfos-mrna-is-an-indirect-marker-of-neuronal-activity\/","og_site_name":"The Kolabtree Blog","article_publisher":"https:\/\/www.facebook.com\/kolabtree","article_published_time":"2021-05-20T04:22:28+00:00","article_modified_time":"2021-05-20T04:40:52+00:00","og_image":[{"width":1078,"height":516,"url":"https:\/\/www.kolabtree.com\/blog\/wp-content\/uploads\/2021\/05\/Blog-headertemplate-1078x516-1.png","type":"image\/png"}],"author":"Ramya Sriram","twitter_card":"summary_large_image","twitter_creator":"@kolabtree","twitter_site":"@kolabtree","twitter_misc":{"Written by":"Ramya Sriram","Est. reading time":"2 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"Article","@id":"https:\/\/www.kolabtree.com\/blog\/de\/cristina-cosi-expression-ofcfos-mrna-is-an-indirect-marker-of-neuronal-activity\/#article","isPartOf":{"@id":"https:\/\/www.kolabtree.com\/blog\/de\/cristina-cosi-expression-ofcfos-mrna-is-an-indirect-marker-of-neuronal-activity\/"},"author":{"name":"Ramya Sriram","@id":"https:\/\/www.kolabtree.com\/blog\/#\/schema\/person\/81992f5863a1b06d132a47822e7b4400"},"headline":"Cristina Cosi: Expression of c-fos mRNA is an indirect marker of neuronal activity","datePublished":"2021-05-20T04:22:28+00:00","dateModified":"2021-05-20T04:40:52+00:00","mainEntityOfPage":{"@id":"https:\/\/www.kolabtree.com\/blog\/de\/cristina-cosi-expression-ofcfos-mrna-is-an-indirect-marker-of-neuronal-activity\/"},"wordCount":282,"commentCount":0,"publisher":{"@id":"https:\/\/www.kolabtree.com\/blog\/#organization"},"articleSection":["Freelancer Showcase"],"inLanguage":"en-US","potentialAction":[{"@type":"CommentAction","name":"Comment","target":["https:\/\/www.kolabtree.com\/blog\/de\/cristina-cosi-expression-ofcfos-mrna-is-an-indirect-marker-of-neuronal-activity\/#respond"]}]},{"@type":"WebPage","@id":"https:\/\/www.kolabtree.com\/blog\/de\/cristina-cosi-expression-ofcfos-mrna-is-an-indirect-marker-of-neuronal-activity\/","url":"https:\/\/www.kolabtree.com\/blog\/de\/cristina-cosi-expression-ofcfos-mrna-is-an-indirect-marker-of-neuronal-activity\/","name":"Cristina Cosi: Expression of c-fos mRNA is an indirect marker of neuronal activity","isPartOf":{"@id":"https:\/\/www.kolabtree.com\/blog\/#website"},"datePublished":"2021-05-20T04:22:28+00:00","dateModified":"2021-05-20T04:40:52+00:00","description":"Neuroscientist Cristina Cosi's research publication on the expression of c-fos mRNA as an indirect marker of neuronal activity.","breadcrumb":{"@id":"https:\/\/www.kolabtree.com\/blog\/de\/cristina-cosi-expression-ofcfos-mrna-is-an-indirect-marker-of-neuronal-activity\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/www.kolabtree.com\/blog\/de\/cristina-cosi-expression-ofcfos-mrna-is-an-indirect-marker-of-neuronal-activity\/"]}]},{"@type":"BreadcrumbList","@id":"https:\/\/www.kolabtree.com\/blog\/de\/cristina-cosi-expression-ofcfos-mrna-is-an-indirect-marker-of-neuronal-activity\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/www.kolabtree.com\/blog\/"},{"@type":"ListItem","position":2,"name":"Cristina Cosi: Expression of c-fos mRNA is an indirect marker of neuronal activity"}]},{"@type":"WebSite","@id":"https:\/\/www.kolabtree.com\/blog\/#website","url":"https:\/\/www.kolabtree.com\/blog\/","name":"The Kolabtree Blog","description":"Expert Views on Science, Innovation and Product Development","publisher":{"@id":"https:\/\/www.kolabtree.com\/blog\/#organization"},"potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/www.kolabtree.com\/blog\/?s={search_term_string}"},"query-input":"required name=search_term_string"}],"inLanguage":"en-US"},{"@type":"Organization","@id":"https:\/\/www.kolabtree.com\/blog\/#organization","name":"Kolabtree","url":"https:\/\/www.kolabtree.com\/blog\/","logo":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/www.kolabtree.com\/blog\/#\/schema\/logo\/image\/","url":"","contentUrl":"","caption":"Kolabtree"},"image":{"@id":"https:\/\/www.kolabtree.com\/blog\/#\/schema\/logo\/image\/"},"sameAs":["https:\/\/www.facebook.com\/kolabtree","https:\/\/twitter.com\/kolabtree","https:\/\/instagram.com\/kolabtree","https:\/\/www.linkedin.com\/company\/kolabtree","https:\/\/en.m.wikipedia.org\/wiki\/Kolabtree"]},{"@type":"Person","@id":"https:\/\/www.kolabtree.com\/blog\/#\/schema\/person\/81992f5863a1b06d132a47822e7b4400","name":"Ramya Sriram","image":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/www.kolabtree.com\/blog\/#\/schema\/person\/image\/","url":"https:\/\/secure.gravatar.com\/avatar\/8100b45c960ebbbbe420e8b3f250515f?s=96&d=mm&r=g","contentUrl":"https:\/\/secure.gravatar.com\/avatar\/8100b45c960ebbbbe420e8b3f250515f?s=96&d=mm&r=g","caption":"Ramya Sriram"},"description":"Ramya Sriram manages digital content and communications at Kolabtree (kolabtree.com), the world's largest freelancing platform for scientists. She has over a decade of experience in publishing, advertising and digital content creation.","url":"https:\/\/www.kolabtree.com\/blog\/author\/ramyas\/"}]}},"amp_enabled":true,"_links":{"self":[{"href":"https:\/\/www.kolabtree.com\/blog\/wp-json\/wp\/v2\/posts\/9141"}],"collection":[{"href":"https:\/\/www.kolabtree.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.kolabtree.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.kolabtree.com\/blog\/wp-json\/wp\/v2\/users\/12"}],"replies":[{"embeddable":true,"href":"https:\/\/www.kolabtree.com\/blog\/wp-json\/wp\/v2\/comments?post=9141"}],"version-history":[{"count":2,"href":"https:\/\/www.kolabtree.com\/blog\/wp-json\/wp\/v2\/posts\/9141\/revisions"}],"predecessor-version":[{"id":9146,"href":"https:\/\/www.kolabtree.com\/blog\/wp-json\/wp\/v2\/posts\/9141\/revisions\/9146"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.kolabtree.com\/blog\/wp-json\/wp\/v2\/media\/9147"}],"wp:attachment":[{"href":"https:\/\/www.kolabtree.com\/blog\/wp-json\/wp\/v2\/media?parent=9141"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.kolabtree.com\/blog\/wp-json\/wp\/v2\/categories?post=9141"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.kolabtree.com\/blog\/wp-json\/wp\/v2\/tags?post=9141"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}