{"id":9141,"date":"2021-05-20T04:22:28","date_gmt":"2021-05-20T04:22:28","guid":{"rendered":"https:\/\/www.kolabtree.com\/blog\/?p=9141"},"modified":"2021-05-20T04:40:52","modified_gmt":"2021-05-20T04:40:52","slug":"cristina-cosi-expression-ofcfos-mrna-is-an-indirect-marker-of-neuronal-activity","status":"publish","type":"post","link":"https:\/\/www.kolabtree.com\/blog\/pt\/cristina-cosi-expression-ofcfos-mrna-is-an-indirect-marker-of-neuronal-activity\/","title":{"rendered":"Cristina Cosi: A express\u00e3o de c-fos mRNA \u00e9 um marcador indireto da atividade neuronal"},"content":{"rendered":"

A express\u00e3o do \"gene precoce imediato\" c-<\/span>fos<\/span><\/i> \u00e9 um marcador indireto da atividade neuronal porque o c-fos \u00e9 freq\u00fcentemente expresso quando os neur\u00f4nios disparam potenciais de a\u00e7\u00e3o. c-<\/span>fos<\/span><\/i> A express\u00e3o do mRNA est\u00e1 correlacionada com a neurotransmiss\u00e3o glutam\u00e1trica e tem sido estudada extensivamente em psicofarmacologia. O RNAscope (ACD Bio) \u00e9 uma tecnologia patenteada de detec\u00e7\u00e3o de mRNA in situ (1) que utiliza amplifica\u00e7\u00e3o ramificada de DNA e sondas z pareadas para fornecer um ensaio robusto e espec\u00edfico, projetado principalmente para uso em se\u00e7\u00f5es fixas de parafina formalina. A tecnologia, adaptada \u00e0s se\u00e7\u00f5es congeladas do tecido cerebral, permitiu o estudo da modula\u00e7\u00e3o do c-<\/span>fos<\/span><\/i> express\u00e3o em diferentes neurocircuitos por um antagonista D3 preferencial, potencial antipsic\u00f3tico, em ratos tratados com o bloqueador de canal receptor de glutamato NMDA MK-801 (2). O m\u00e9todo e sua valida\u00e7\u00e3o s\u00e3o descritos em Cosi et al,<\/span>https:\/\/doi.org\/10.1016\/j.mex.2021.101348<\/span><\/a><\/p>\n

[1] F. Wang, J. Flanagan, N. Su, L.-C. Wang, S. Bui, A. Nielson, X. Wu, H.-T. Vo, X.-J. Ma, Y. Luo, RNAscope\u00ae: Uma nova plataforma de an\u00e1lise in situ de RNA para tecidos fixados em parafina, J. Mol. Diagn. 14 (2012) 22-29, doi:<\/span>10.1016\/j.jmoldx.2011.08.002.<\/span><\/p>\n

[2] C. Cosi, J.C. Martel, A.L. Auclair, G. Collo, L. Cavalleri, P. Heusler, L. Leriche, F. Gaudoux, P. Sokoloff, P.C. Moser, S. GattiMcArthur, Perfil farmacol\u00f3gico de F17464, uma Dopamina D<\/span>3 <\/span>Antagonista preferencial do receptor, Eur. J. Pharmacol. 890 (2021) 173635 <\/span>https:\/\/doi.org\/10.1016\/j.ejphar.2020.173635.<\/span><\/p>\n

Sobre o autor<\/strong><\/p>\n

\"\"<\/a><\/p>\n

Cristina Cosi<\/a>A Kolabtree, MSc, PhD, bi\u00f3logo e farmacologista, neurocientista por forma\u00e7\u00e3o, \u00e9 consultor cient\u00edfico freelancer na Kolabtree.\u00a0<\/span><\/p>\n

Ela dedicou sua carreira \u00e0 compreens\u00e3o dos mecanismos da plasticidade neuronal e \u00e0 descoberta de drogas que poderiam ser ben\u00e9ficas contra doen\u00e7as neurol\u00f3gicas (incluindo Parkinson) e neuropsiqui\u00e1tricas e a dor.<\/span><\/p><\/blockquote>","protected":false},"excerpt":{"rendered":"

The expression of the \u201cimmediate early gene\u201d c-fos is an indirect marker of neuronal activity because c-fos is often expressed when neurons fire action potentials. c-fos mRNA expression is correlated to glutamatergic neurotransmission and has been studied extensively in psychopharmacology. RNAscope (ACD Bio) is a patented in situ mRNA detection technology (1) that uses branched<\/p>\n

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