{"id":2581,"date":"2017-11-22T09:03:01","date_gmt":"2017-11-22T09:03:01","guid":{"rendered":"https:\/\/blog.kolabtree.com\/?p=2581"},"modified":"2021-09-27T08:58:38","modified_gmt":"2021-09-27T08:58:38","slug":"tip-enhanced-raman-spectroscopy","status":"publish","type":"post","link":"https:\/\/www.kolabtree.com\/blog\/pt\/tip-enhanced-raman-spectroscopy\/","title":{"rendered":"Dica Espectroscopia Raman melhorada: Transformando as Ci\u00eancias Anal\u00edticas"},"content":{"rendered":"

\"Ver para crer\" \u00e9 uma frase comumente usada nos corredores cient\u00edficos. A microscopia, portanto, \u00e9 um ramo magn\u00edfico que, na verdade, equipam os pesquisadores com o poder de visualizar as coisas de uma maneira que antes se pensava ser imposs\u00edvel. Apesar dos sofismas envolvidos na interpreta\u00e7\u00e3o de muitas das imagens obtidas atrav\u00e9s da microscopia, tanto os cientistas quanto o p\u00fablico em geral, sempre caem em evid\u00eancia visual que detalha os meandros de uma c\u00e9lula ou uma mol\u00e9cula ou uma nanopart\u00edcula. O advento de t\u00e9cnicas de microscopia baseadas em el\u00e9trons como a microscopia de varredura de t\u00faneis capacitou ainda mais os pesquisadores a mergulharem no mundo dos \u00e1tomos. Em outras palavras, estas t\u00e9cnicas tornaram poss\u00edvel at\u00e9 mesmo a observa\u00e7\u00e3o de mol\u00e9culas com resolu\u00e7\u00e3o at\u00f4mica.<\/p>\n

O encanto de uma imagem detalhando os componentes de um sistema complicado, digamos uma mol\u00e9cula de DNA, \u00e9 duradouro, por\u00e9m o lado negativo \u00e9 que a caracteriza\u00e7\u00e3o espectrosc\u00f3pica da amostra \u00e9 essencial para entender os eventos que ocorrem na escala microsc\u00f3pica ou nanosc\u00f3pica. A espectroscopia e a microscopia devem, portanto, andar de m\u00e3os dadas, e os cientistas j\u00e1 em 1985 sugeriram combinar t\u00e9cnicas de sondagem com m\u00e9todos como Raman ou espectroscopia de infravermelho para obter informa\u00e7\u00f5es simult\u00e2neas da topografia, bem como da natureza qu\u00edmica. Dica Espectroscopia Raman Avan\u00e7ada<\/strong>O TERS, popularmente abreviado como TERS, \u00e9 um desses m\u00e9todos que combina Microscopia de For\u00e7a At\u00f4mica ou STM com Espectroscopia Raman. Esta combina\u00e7\u00e3o \u00fanica d\u00e1 ao cientista anal\u00edtico a capacidade de olhar para a superf\u00edcie em detalhes \u00f3pticos, topogr\u00e1ficos e qu\u00edmicos. Anteriormente, pensava-se ser imposs\u00edvel visualizar um objeto com dimens\u00f5es inferiores \u00e0 metade do comprimento de onda da luz. Este limite \u00e9 geralmente conhecido como o limite Abbe. TERS, entretanto, quebra este limite tornando poss\u00edvel a resolu\u00e7\u00e3o a n\u00edvel molecular com a vantagem adicional da informa\u00e7\u00e3o qu\u00edmica. O mecanismo em uma casca de noz \u00e9 mostrado no esquema.<\/p>\n

\"\"
\nEsquema de um esquema de TERS empregado para an\u00e1lise de \u00e1cido nucl\u00e9ico. Reproduzido a partir da Refer\u00eancia <\/em>(link)<\/em><\/a><\/p>\n

Na tecnologia TERS, um campo nanom\u00e9trico localmente melhorado na vizinhan\u00e7a imediata do \u00e1pice da ponta metalizada \u00e9 gerado sob a ilumina\u00e7\u00e3o incidente da luz laser como uma fonte de excita\u00e7\u00e3o local na escala nanom\u00e9trica. Ele pode ser usado para excitar localmente o sinal \u00f3ptico da amostra e produzir a dispers\u00e3o Raman apenas a partir do nano-volume nas proximidades do \u00e1pice da ponta. O \u00e1pice da ponta \u00e9 o dispositivo chave em TERS como uma dispers\u00e3o local, que real\u00e7a e dispersa o campo evanescente localizado para o campo distante a ser coletado e detectado. Ele garante a alta melhoria e resolu\u00e7\u00e3o para al\u00e9m do limite de difra\u00e7\u00e3o. Em ess\u00eancia, o TERS \u00e9 capaz de gravar imagens de resolu\u00e7\u00e3o submolecular que cont\u00eam a assinatura espectral de cada ponto sondado pela ponta.<\/span><\/p>\n

Prof. Volker Deckert<\/a> e colegas de trabalho (da Universidade Friedrich Schiller Jena, Alemanha) usaram TERS para sequenciar uma mol\u00e9cula de RNA. A aplica\u00e7\u00e3o do TERS aos \u00e1cidos nucl\u00e9icos tem sido uma revela\u00e7\u00e3o, pois permite a resolu\u00e7\u00e3o de nucleobases individuais bem espa\u00e7adas (ver esquema). Outro grupo na ETH Zurique liderado por Prof. Renato Zenobi<\/a> tem caracterizado extensivamente os espectros de DNA Raman. Estes estudos podem agora potencialmente levar a compreender as intera\u00e7\u00f5es do DNA com mol\u00e9culas como prote\u00ednas, drogas anticancer\u00edgenas, etc. Em outro relat\u00f3rio<\/a>O estudo TERS do Amyloid \u03b2, (um derivado proteol\u00edtico secretado da prote\u00edna precursora do amil\u00f3ide que \u00e9 um fator cr\u00edtico na \"falha sin\u00e1ptica\" inicial que \u00e9 observada na patog\u00eanese da doen\u00e7a de Alzheimer) foi realizado, mostrando assim a imensa aplicabilidade do TERS a dom\u00ednios inexplorados da biologia.<\/p>\n

Apesar dos desafios envolvidos em tornar a TERS uma t\u00e9cnica anal\u00edtica de rotina, seu futuro para os estudos biol\u00f3gicos est\u00e1 mais brilhante do que nunca. Para concluir, a linha proverbial \"Uma imagem vale por 1000 palavras\"<\/em>parece apta para uma imagem TERS, com a ligeira modifica\u00e7\u00e3o que \"Uma imagem TERS vale mais que 1000 espectros carregados com informa\u00e7\u00f5es qu\u00edmicas inestim\u00e1veis\".<\/em>.<\/p>\n

————————-
\nConsulte Sugosh Prabhu<\/a> para um projeto ou falar com outros especialistas em espectroscopia Kolabtree aqui<\/a>.<\/p>","protected":false},"excerpt":{"rendered":"

\u201cSeeing is believing\u201d is often a commonly used phrase in the scientific corridors. Microscopy therefore, is a magnificent branch that actually equips researchers with the power to visualize things in a manner that was previously thought to be impossible. Despite the sophistry involved with the interpretation of many of the images obtained through microscopy, scientists<\/p>\n

Leia mais<\/a><\/div>","protected":false},"author":36,"featured_media":2694,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[442],"tags":[],"acf":[],"yoast_head":"\nTip Enhanced Raman Spectroscopy - Transforming Analytical Sciences<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.kolabtree.com\/blog\/pt\/tip-enhanced-raman-spectroscopy\/\" \/>\n<meta property=\"og:locale\" content=\"pt_BR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Tip Enhanced Raman Spectroscopy: Transforming Analytical Sciences\" \/>\n<meta property=\"og:description\" content=\"\u201cSeeing is believing\u201d is often a commonly used phrase in the scientific corridors. 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