{"id":9141,"date":"2021-05-20T04:22:28","date_gmt":"2021-05-20T04:22:28","guid":{"rendered":"https:\/\/www.kolabtree.com\/blog\/?p=9141"},"modified":"2021-05-20T04:40:52","modified_gmt":"2021-05-20T04:40:52","slug":"cristina-cosi-expression-ofcfos-mrna-is-an-indirect-marker-of-neuronal-activity","status":"publish","type":"post","link":"https:\/\/www.kolabtree.com\/blog\/ja\/cristina-cosi-expression-ofcfos-mrna-is-an-indirect-marker-of-neuronal-activity\/","title":{"rendered":"\u30af\u30ea\u30b9\u30c6\u30a3\u30fc\u30ca\u30fb\u30b3\u30fc\u30b8c-fos mRNA\u306e\u767a\u73fe\u306f\u3001\u795e\u7d4c\u7d30\u80de\u6d3b\u6027\u306e\u9593\u63a5\u7684\u306a\u30de\u30fc\u30ab\u30fc\u3067\u3042\u308b"},"content":{"rendered":"

\u5373\u6642\u578b\u521d\u671f\u907a\u4f1d\u5b50 \"c-\u306e\u767a\u73fe\u3092\u6291\u5236\u3059\u308b\u3002<\/span>\u30d5\u30a9\u30b9<\/span><\/i> \u306f\u3001\u795e\u7d4c\u7d30\u80de\u304c\u6d3b\u52d5\u96fb\u4f4d\u3092\u767a\u706b\u3055\u305b\u308b\u3068\u304d\u306b\u3057\u3070\u3057\u3070\u767a\u73fe\u3059\u308b\u305f\u3081\u3001\u795e\u7d4c\u7d30\u80de\u6d3b\u52d5\u306e\u9593\u63a5\u7684\u306a\u30de\u30fc\u30ab\u30fc\u3068\u306a\u308b\u3002<\/span>\u30d5\u30a9\u30b9<\/span><\/i> mRNA \u306e\u767a\u73fe\u306f\u30b0\u30eb\u30bf\u30df\u30f3\u9178\u4f5c\u52d5\u6027\u795e\u7d4c\u4f1d\u9054\u3068\u76f8\u95a2\u3057\u3066\u304a\u308a\u3001\u7cbe\u795e\u85ac\u7406\u5b66\u306b\u304a\u3044\u3066\u5e83\u7bc4\u306b\u7814\u7a76\u3055\u308c\u3066\u3044\u307e\u3059\u3002RNAscope\uff08ACD\u30d0\u30a4\u30aa\u793e\uff09\u306f\u3001\u5206\u5c90\u578bDNA\u5897\u5e45\u3068z\u30da\u30a2\u30d7\u30ed\u30fc\u30d6\u3092\u7528\u3044\u305fin situ mRNA\u691c\u51fa\u6280\u8853\uff08\u7279\u8a31\u53d6\u5f97\u6e08\u307f\uff09\uff081\uff09\u3067\u3001\u4e3b\u306b\u30db\u30eb\u30de\u30ea\u30f3\u56fa\u5b9a\u30d1\u30e9\u30d5\u30a3\u30f3\u5207\u7247\u3067\u306e\u4f7f\u7528\u3092\u60f3\u5b9a\u3057\u305f\u5805\u7262\u304b\u3064\u7279\u7570\u7684\u306a\u30a2\u30c3\u30bb\u30a4\u3092\u63d0\u4f9b\u3059\u308b\u3082\u306e\u3067\u3059\u3002\u3053\u306e\u6280\u8853\u3092\u51cd\u7d50\u8133\u7d44\u7e54\u5207\u7247\u306b\u9069\u7528\u3059\u308b\u3053\u3068\u3067\u3001c-sequence\u306e\u5909\u8abf\u3092\u7814\u7a76\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u306b\u306a\u308a\u307e\u3057\u305f\u3002<\/span>\u30d5\u30a9\u30b9<\/span><\/i> NMDA\u30b0\u30eb\u30bf\u30df\u30f3\u9178\u53d7\u5bb9\u4f53\u30c1\u30e3\u30cd\u30eb\u906e\u65ad\u85acMK-801\u3067\u6cbb\u7642\u3057\u305f\u30de\u30a6\u30b9\u306b\u304a\u3044\u3066\u3001\u512a\u5148\u7684D3\u30a2\u30f3\u30bf\u30b4\u30cb\u30b9\u30c8\u3001\u6f5c\u5728\u7684\u6297\u7cbe\u795e\u75c5\u85ac\u306b\u3088\u3063\u3066\u7570\u306a\u308b\u795e\u7d4c\u56de\u8def\u306b\u304a\u3051\u308b\u767a\u73fe\u3092\u6e2c\u5b9a\u3057\u305f(2)\u3002\u3053\u306e\u65b9\u6cd5\u3068\u305d\u306e\u691c\u8a3c\u306f\u3001Cosi et al.\u306b\u8a18\u8f09\u3055\u308c\u3066\u3044\u307e\u3059\u3002<\/span>https:\/\/doi.org\/10.1016\/j.mex.2021.101348<\/span><\/a><\/p>\n

[1] F. Wang, J. Flanagan, N. Su, L.-C.Wang, S. Bui, A. Nielson, X. Wu, H.-T.Luo, X.-J. Ma, Y. Luo, RNAscope\u00ae:\u3092\u7528\u3044\u305f\u3001\u30db\u30eb\u30de\u30ea\u30f3\u56fa\u5b9a\u30d1\u30e9\u30d5\u30a3\u30f3\u5305\u57cb\u7d44\u7e54\u306e\u65b0\u3057\u3044 in situ RNA \u89e3\u6790\u30d7\u30e9\u30c3\u30c8\u30d5\u30a9\u30fc\u30e0\u3001J. Mol.Diagn.14 (2012) 22-29, doi:<\/span>10.1016\/j.jmoldx.2011.08.002.<\/span><\/p>\n

[2] C. Cosi, J.C. Martel, A.L. Auclair, G. Collo, L. Cavalleri, P. Heusler, L. Leriche, F. Gaudoux, P. Sokoloff, P.C. Moser, S. GattiMcArthur, Pharmacology Profile of F17464, a Dopamine D. Coupling, D. A. C. Auclair, L. Cavalleri, L. Auclair, G. Collo<\/span>3 <\/span>\u53d7\u5bb9\u4f53\u512a\u5148\u306e\u30a2\u30f3\u30bf\u30b4\u30cb\u30b9\u30c8\u3001Eur.J. Pharmacol.890 (2021) 173635 <\/span>https:\/\/doi.org\/10.1016\/j.ejphar.2020.173635.<\/span><\/p>\n

\u8457\u8005\u306b\u3064\u3044\u3066<\/strong><\/p>\n

\"\"<\/a><\/p>\n

\u30af\u30ea\u30b9\u30c6\u30a3\u30fc\u30ca\u30fb\u30b3\u30fc\u30b8<\/a>\u751f\u7269\u5b66\u8005\u3001\u85ac\u7406\u5b66\u8005\u3001\u795e\u7d4c\u79d1\u5b66\u8005\u3001Kolabtree\u306e\u30d5\u30ea\u30fc\u30e9\u30f3\u30b9\u306e\u79d1\u5b66\u30b3\u30f3\u30b5\u30eb\u30bf\u30f3\u30c8\u3002\u00a0<\/span><\/p>\n

\u795e\u7d4c\u53ef\u5851\u6027\u306e\u30e1\u30ab\u30cb\u30ba\u30e0\u306e\u89e3\u660e\u3068\u3001\u795e\u7d4c\u75be\u60a3\uff08\u30d1\u30fc\u30ad\u30f3\u30bd\u30f3\u75c5\u3092\u542b\u3080\uff09\u3001\u7cbe\u795e\u795e\u7d4c\u75be\u60a3\u3001\u75bc\u75db\u306b\u6709\u52b9\u306a\u85ac\u7269\u306e\u767a\u898b\u306b\u5c3d\u529b\u3057\u3066\u3044\u308b\u3002<\/span><\/p><\/blockquote>","protected":false},"excerpt":{"rendered":"

The expression of the \u201cimmediate early gene\u201d c-fos is an indirect marker of neuronal activity because c-fos is often expressed when neurons fire action potentials. c-fos mRNA expression is correlated to glutamatergic neurotransmission and has been studied extensively in psychopharmacology. RNAscope (ACD Bio) is a patented in situ mRNA detection technology (1) that uses branched DNA amplification and z paired probes\u00a0 to deliver a robust and specific assay, designed primarily for use on formalin fixed paraffin sections. The technology, adapted to frozen brain tissue sections, allowed the study of the modulation of c-fos expression in different neurocircuitries by a preferential D3 antagonist, potential antipsychotic, in mice treated with the NMDA<\/p>\n

\u7d9a\u304d\u3092\u8aad\u3080<\/a><\/div>","protected":false},"author":12,"featured_media":9147,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[662],"tags":[],"acf":[],"yoast_head":"\nCristina Cosi: Expression of c-fos mRNA is an indirect marker of neuronal activity<\/title>\n<meta name=\"description\" content=\"Neuroscientist Cristina Cosi's research publication on the expression of c-fos mRNA as an indirect marker of neuronal activity.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.kolabtree.com\/blog\/ja\/cristina-cosi-expression-ofcfos-mrna-is-an-indirect-marker-of-neuronal-activity\/\" \/>\n<meta property=\"og:locale\" content=\"ja_JP\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Cristina Cosi: Expression of c-fos mRNA is an indirect marker 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