{"id":9141,"date":"2021-05-20T04:22:28","date_gmt":"2021-05-20T04:22:28","guid":{"rendered":"https:\/\/www.kolabtree.com\/blog\/?p=9141"},"modified":"2021-05-20T04:40:52","modified_gmt":"2021-05-20T04:40:52","slug":"cristina-cosi-expression-ofcfos-mrna-is-an-indirect-marker-of-neuronal-activity","status":"publish","type":"post","link":"https:\/\/www.kolabtree.com\/blog\/it\/cristina-cosi-expression-ofcfos-mrna-is-an-indirect-marker-of-neuronal-activity\/","title":{"rendered":"Cristina Cosi: L'espressione di c-fos mRNA \u00e8 un marcatore indiretto di attivit\u00e0 neuronale"},"content":{"rendered":"

L'espressione del \"gene precoce immediato\" c-<\/span>fos<\/span><\/i> \u00e8 un marcatore indiretto di attivit\u00e0 neuronale perch\u00e9 c-fos \u00e8 spesso espresso quando i neuroni sparano potenziali d'azione. c-<\/span>fos<\/span><\/i> L'espressione dell'mRNA \u00e8 correlata alla neurotrasmissione glutammatergica ed \u00e8 stata ampiamente studiata in psicofarmacologia. RNAscope (ACD Bio) \u00e8 una tecnologia brevettata di rilevamento dell'mRNA in situ (1) che utilizza un'amplificazione ramificata del DNA e sonde accoppiate a z per fornire un test robusto e specifico, progettato principalmente per l'uso su sezioni di paraffina fissate in formalina. La tecnologia, adattata a sezioni di tessuto cerebrale congelato, ha permesso lo studio della modulazione di c-<\/span>fos<\/span><\/i> espressione in diversi neurocircuiti da un antagonista D3 preferenziale, potenziale antipsicotico, in topi trattati con il bloccante del canale del recettore del glutammato NMDA MK-801 (2). Il metodo e la sua convalida sono descritti in Cosi et al,<\/span>https:\/\/doi.org\/10.1016\/j.mex.2021.101348<\/span><\/a><\/p>\n

[1] F. Wang, J. Flanagan, N. Su, L.-C. Wang, S. Bui, A. Nielson, X. Wu, H.-T. Vo, X.-J. Ma, Y. Luo, RNAscope\u00ae: Un nuovo in situ RNA piattaforma di analisi per i tessuti fissati in formalina paraffina-embedded, J. Mol. Diagn. 14 (2012) 22-29, doi:<\/span>10.1016\/j.jmoldx.2011.08.002.<\/span><\/p>\n

[2] C. Cosi, J.C. Martel, A.L. Auclair, G. Collo, L. Cavalleri, P. Heusler, L. Leriche, F. Gaudoux, P. Sokoloff, P.C. Moser, S. GattiMcArthur, Pharmacology Profile of F17464, a Dopamine D<\/span>3 <\/span>Antagonista preferenziale del recettore, Eur. J. Pharmacol. 890 (2021) 173635 <\/span>https:\/\/doi.org\/10.1016\/j.ejphar.2020.173635.<\/span><\/p>\n

L'autore<\/strong><\/p>\n

\"\"<\/a><\/p>\n

Cristina Cosi<\/a>, MSc, PhD, biologo e farmacologo, neuroscienziato di formazione, \u00e8 consulente scientifico freelance presso Kolabtree.\u00a0<\/span><\/p>\n

Ha dedicato la sua carriera alla comprensione dei meccanismi della plasticit\u00e0 neuronale e alla scoperta di farmaci che potrebbero essere utili contro le malattie neurologiche (incluso Parkinson) e neuropsichiatriche e il dolore.<\/span><\/p><\/blockquote>","protected":false},"excerpt":{"rendered":"

The expression of the \u201cimmediate early gene\u201d c-fos is an indirect marker of neuronal activity because c-fos is often expressed when neurons fire action potentials. c-fos mRNA expression is correlated to glutamatergic neurotransmission and has been studied extensively in psychopharmacology. RNAscope (ACD Bio) is a patented in situ mRNA detection technology (1) that uses branched<\/p>\n

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