{"id":9141,"date":"2021-05-20T04:22:28","date_gmt":"2021-05-20T04:22:28","guid":{"rendered":"https:\/\/www.kolabtree.com\/blog\/?p=9141"},"modified":"2021-05-20T04:40:52","modified_gmt":"2021-05-20T04:40:52","slug":"cristina-cosi-expression-ofcfos-mrna-is-an-indirect-marker-of-neuronal-activity","status":"publish","type":"post","link":"https:\/\/www.kolabtree.com\/blog\/fr\/cristina-cosi-expression-ofcfos-mrna-is-an-indirect-marker-of-neuronal-activity\/","title":{"rendered":"Cristina Cosi : L'expression de l'ARNm c-fos est un marqueur indirect de l'activit\u00e9 neuronale"},"content":{"rendered":"

L'expression du \"g\u00e8ne pr\u00e9coce imm\u00e9diat\" c-<\/span>fos<\/span><\/i> est un marqueur indirect de l'activit\u00e9 neuronale car c-fos est souvent exprim\u00e9 lorsque les neurones \u00e9mettent des potentiels d'action. c-<\/span>fos<\/span><\/i> L'expression de l'ARNm est corr\u00e9l\u00e9e \u00e0 la neurotransmission glutamatergique et a \u00e9t\u00e9 largement \u00e9tudi\u00e9e en psychopharmacologie. RNAscope (ACD Bio) est une technologie brevet\u00e9e de d\u00e9tection in situ de l'ARNm (1) qui utilise l'amplification de l'ADN ramifi\u00e9 et des sondes appari\u00e9es en Z pour fournir un test robuste et sp\u00e9cifique, con\u00e7u principalement pour \u00eatre utilis\u00e9 sur des coupes de paraffine fix\u00e9es au formol. La technologie, adapt\u00e9e aux coupes de tissus c\u00e9r\u00e9braux congel\u00e9s, a permis l'\u00e9tude de la modulation de l'ARNm c-<\/span>fos<\/span><\/i> dans diff\u00e9rents neurocircuits par un antagoniste D3 pr\u00e9f\u00e9rentiel, antipsychotique potentiel, chez des souris trait\u00e9es par le bloqueur de canal du r\u00e9cepteur du glutamate NMDA MK-801 (2). La m\u00e9thode et sa validation sont d\u00e9crites dans Cosi et al,<\/span>https:\/\/doi.org\/10.1016\/j.mex.2021.101348<\/span><\/a><\/p>\n

1] F. Wang, J. Flanagan, N. Su, L.-C. Wang, S. Bui, A. Nielson, X. Wu, H.-T. Vo, X.-J. Ma, Y. Luo, RNAscope\u00ae : A novel in situ RNA analysis platform for formalin-fixed paraffin-embedded tissues, J. Mol. Diagn. 14 (2012) 22-29, doi :<\/span>10.1016\/j.jmoldx.2011.08.002.<\/span><\/p>\n

2] C. Cosi, J.C. Martel, A.L. Auclair, G. Collo, L. Cavalleri, P. Heusler, L. Leriche, F. Gaudoux, P. Sokoloff, P.C. Moser, S. Gatti-McArthur, Pharmacology Profile of F17464, a Dopamine D<\/span>3 <\/span>Antagoniste pr\u00e9f\u00e9rentiel des r\u00e9cepteurs, Eur. J. Pharmacol. 890 (2021) 173635 <\/span>https:\/\/doi.org\/10.1016\/j.ejphar.2020.173635.<\/span><\/p>\n

A propos de l'auteur<\/strong><\/p>\n

\"\"<\/a><\/p>\n

Cristina Cosi<\/a>MSc, PhD, biologiste et pharmacologue, neuroscientifique de formation, est consultant scientifique ind\u00e9pendant chez Kolabtree.\u00a0<\/span><\/p>\n

Elle a consacr\u00e9 sa carri\u00e8re \u00e0 la compr\u00e9hension des m\u00e9canismes de la plasticit\u00e9 neuronale et \u00e0 la d\u00e9couverte de m\u00e9dicaments qui pourraient \u00eatre b\u00e9n\u00e9fiques contre les maladies neurologiques (notamment Parkinson) et neuropsychiatriques et la douleur.<\/span><\/p><\/blockquote>","protected":false},"excerpt":{"rendered":"

L'expression du \"g\u00e8ne pr\u00e9coce imm\u00e9diat\" c-fos est un marqueur indirect de l'activit\u00e9 neuronale car c-fos est souvent exprim\u00e9 lorsque les neurones \u00e9mettent des potentiels d'action. L'expression de l'ARNm c-fos est corr\u00e9l\u00e9e \u00e0 la neurotransmission glutamatergique et a \u00e9t\u00e9 largement \u00e9tudi\u00e9e en psychopharmacologie. Le RNAscope (ACD Bio) est une technologie brevet\u00e9e de d\u00e9tection in situ de l'ARNm (1) qui utilise des mol\u00e9cules d'ARNm ramifi\u00e9es.<\/p>\n

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