{"id":9141,"date":"2021-05-20T04:22:28","date_gmt":"2021-05-20T04:22:28","guid":{"rendered":"https:\/\/www.kolabtree.com\/blog\/?p=9141"},"modified":"2021-05-20T04:40:52","modified_gmt":"2021-05-20T04:40:52","slug":"cristina-cosi-expression-ofcfos-mrna-is-an-indirect-marker-of-neuronal-activity","status":"publish","type":"post","link":"https:\/\/www.kolabtree.com\/blog\/de\/cristina-cosi-expression-ofcfos-mrna-is-an-indirect-marker-of-neuronal-activity\/","title":{"rendered":"Cristina Cosi: Die Expression von c-fos mRNA ist ein indirekter Marker f\u00fcr neuronale Aktivit\u00e4t"},"content":{"rendered":"

Die Expression des \"unmittelbaren fr\u00fchen Gens\" c-<\/span>fos<\/span><\/i> ist ein indirekter Marker f\u00fcr neuronale Aktivit\u00e4t, da c-fos h\u00e4ufig exprimiert wird, wenn Neuronen Aktionspotenziale ausl\u00f6sen. c-<\/span>fos<\/span><\/i> Die mRNA-Expression steht in Zusammenhang mit der glutamatergen Neurotransmission und wurde in der Psychopharmakologie eingehend untersucht. RNAscope (ACD Bio) ist eine patentierte Technologie zum In-situ-MRNA-Nachweis (1), die eine verzweigte DNA-Amplifikation und z-gepaarte Sonden verwendet, um einen robusten und spezifischen Assay zu liefern, der in erster Linie f\u00fcr die Verwendung an formalinfixierten Paraffinschnitten konzipiert wurde. Die Technologie wurde an gefrorene Hirngewebeschnitte angepasst und erm\u00f6glichte die Untersuchung der Modulation von c-<\/span>fos<\/span><\/i> Expression in verschiedenen Neurokreisl\u00e4ufen durch einen bevorzugten D3-Antagonisten, ein potenzielles Antipsychotikum, bei M\u00e4usen, die mit dem NMDA-Glutamatrezeptor-Kanalblocker MK-801 behandelt wurden (2). Die Methode und ihre Validierung sind in Cosi et al. beschrieben,<\/span>https:\/\/doi.org\/10.1016\/j.mex.2021.101348<\/span><\/a><\/p>\n

[1] F. Wang, J. Flanagan, N. Su, L.-C. Wang, S. Bui, A. Nielson, X. Wu, H.-T. Vo, X.-J. Ma, Y. Luo, RNAscope\u00ae: A novel in situ RNA analysis platform for formalin-fixed paraffin-embedded tissues, J. Mol. Diagn. 14 (2012) 22-29, doi:<\/span>10.1016\/j.jmoldx.2011.08.002.<\/span><\/p>\n

[2] C. Cosi, J.C. Martel, A.L. Auclair, G. Collo, L. Cavalleri, P. Heusler, L. Leriche, F. Gaudoux, P. Sokoloff, P.C. Moser, S. GattiMcArthur, Pharmacology Profile of F17464, a Dopamine D<\/span>3 <\/span>Bevorzugter Rezeptor-Antagonist, Eur. J. Pharmacol. 890 (2021) 173635 <\/span>https:\/\/doi.org\/10.1016\/j.ejphar.2020.173635.<\/span><\/p>\n

\u00dcber den Autor<\/strong><\/p>\n

\"\"<\/a><\/p>\n

Cristina Cosi<\/a>MSc, PhD, Biologe und Pharmakologe, ausgebildeter Neurowissenschaftler, ist freiberuflicher wissenschaftlicher Berater bei Kolabtree.\u00a0<\/span><\/p>\n

Sie hat ihre Karriere dem Verst\u00e4ndnis der Mechanismen der neuronalen Plastizit\u00e4t und der Entdeckung von Medikamenten gewidmet, die bei neurologischen (einschlie\u00dflich Parkinson) und neuropsychiatrischen Erkrankungen sowie bei Schmerzen hilfreich sein k\u00f6nnten.<\/span><\/p><\/blockquote>","protected":false},"excerpt":{"rendered":"

The expression of the \u201cimmediate early gene\u201d c-fos is an indirect marker of neuronal activity because c-fos is often expressed when neurons fire action potentials. c-fos mRNA expression is correlated to glutamatergic neurotransmission and has been studied extensively in psychopharmacology. RNAscope (ACD Bio) is a patented in situ mRNA detection technology (1) that uses branched<\/p>\n

Mehr lesen<\/a><\/div>","protected":false},"author":12,"featured_media":9147,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[662],"tags":[],"acf":[],"yoast_head":"\nCristina Cosi: Expression of c-fos mRNA is an indirect marker of neuronal activity<\/title>\n<meta name=\"description\" content=\"Neuroscientist Cristina Cosi's research publication on the expression of c-fos mRNA as an indirect marker of neuronal activity.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.kolabtree.com\/blog\/de\/cristina-cosi-expression-ofcfos-mrna-is-an-indirect-marker-of-neuronal-activity\/\" \/>\n<meta property=\"og:locale\" content=\"de_DE\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Cristina Cosi: Expression of c-fos mRNA is an indirect marker of neuronal activity\" \/>\n<meta property=\"og:description\" content=\"Neuroscientist Cristina Cosi's research publication on the expression of c-fos mRNA as an indirect marker of neuronal activity.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.kolabtree.com\/blog\/de\/cristina-cosi-expression-ofcfos-mrna-is-an-indirect-marker-of-neuronal-activity\/\" \/>\n<meta property=\"og:site_name\" content=\"The Kolabtree Blog\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/kolabtree\" \/>\n<meta property=\"article:published_time\" content=\"2021-05-20T04:22:28+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2021-05-20T04:40:52+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.kolabtree.com\/blog\/wp-content\/uploads\/2021\/05\/Blog-headertemplate-1078x516-1.png\" \/>\n\t<meta property=\"og:image:width\" content=\"1078\" \/>\n\t<meta property=\"og:image:height\" content=\"516\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/png\" \/>\n<meta name=\"author\" content=\"Ramya Sriram\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:creator\" content=\"@kolabtree\" \/>\n<meta name=\"twitter:site\" content=\"@kolabtree\" \/>\n<meta name=\"twitter:label1\" content=\"Verfasst von\" \/>\n\t<meta name=\"twitter:data1\" content=\"Ramya Sriram\" \/>\n\t<meta name=\"twitter:label2\" content=\"Gesch\u00e4tzte Lesezeit\" \/>\n\t<meta name=\"twitter:data2\" content=\"2\u00a0Minuten\" \/>\n<!-- \/ Yoast SEO Premium plugin. -->","yoast_head_json":{"title":"Cristina Cosi: Expression of c-fos mRNA is an indirect marker of neuronal activity","description":"Neuroscientist Cristina Cosi's research publication on the expression of c-fos mRNA as an indirect marker of neuronal activity.","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.kolabtree.com\/blog\/de\/cristina-cosi-expression-ofcfos-mrna-is-an-indirect-marker-of-neuronal-activity\/","og_locale":"de_DE","og_type":"article","og_title":"Cristina Cosi: Expression of c-fos mRNA is an indirect marker of neuronal activity","og_description":"Neuroscientist Cristina Cosi's research publication on the expression of c-fos mRNA as an indirect marker of neuronal activity.","og_url":"https:\/\/www.kolabtree.com\/blog\/de\/cristina-cosi-expression-ofcfos-mrna-is-an-indirect-marker-of-neuronal-activity\/","og_site_name":"The Kolabtree Blog","article_publisher":"https:\/\/www.facebook.com\/kolabtree","article_published_time":"2021-05-20T04:22:28+00:00","article_modified_time":"2021-05-20T04:40:52+00:00","og_image":[{"width":1078,"height":516,"url":"https:\/\/www.kolabtree.com\/blog\/wp-content\/uploads\/2021\/05\/Blog-headertemplate-1078x516-1.png","type":"image\/png"}],"author":"Ramya Sriram","twitter_card":"summary_large_image","twitter_creator":"@kolabtree","twitter_site":"@kolabtree","twitter_misc":{"Verfasst von":"Ramya Sriram","Gesch\u00e4tzte Lesezeit":"2\u00a0Minuten"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"Article","@id":"https:\/\/www.kolabtree.com\/blog\/de\/cristina-cosi-expression-ofcfos-mrna-is-an-indirect-marker-of-neuronal-activity\/#article","isPartOf":{"@id":"https:\/\/www.kolabtree.com\/blog\/de\/cristina-cosi-expression-ofcfos-mrna-is-an-indirect-marker-of-neuronal-activity\/"},"author":{"name":"Ramya Sriram","@id":"https:\/\/www.kolabtree.com\/blog\/#\/schema\/person\/81992f5863a1b06d132a47822e7b4400"},"headline":"Cristina Cosi: Expression of c-fos mRNA is an indirect marker of neuronal activity","datePublished":"2021-05-20T04:22:28+00:00","dateModified":"2021-05-20T04:40:52+00:00","mainEntityOfPage":{"@id":"https:\/\/www.kolabtree.com\/blog\/de\/cristina-cosi-expression-ofcfos-mrna-is-an-indirect-marker-of-neuronal-activity\/"},"wordCount":282,"commentCount":0,"publisher":{"@id":"https:\/\/www.kolabtree.com\/blog\/#organization"},"articleSection":["Freelancer Showcase"],"inLanguage":"de","potentialAction":[{"@type":"CommentAction","name":"Comment","target":["https:\/\/www.kolabtree.com\/blog\/de\/cristina-cosi-expression-ofcfos-mrna-is-an-indirect-marker-of-neuronal-activity\/#respond"]}]},{"@type":"WebPage","@id":"https:\/\/www.kolabtree.com\/blog\/de\/cristina-cosi-expression-ofcfos-mrna-is-an-indirect-marker-of-neuronal-activity\/","url":"https:\/\/www.kolabtree.com\/blog\/de\/cristina-cosi-expression-ofcfos-mrna-is-an-indirect-marker-of-neuronal-activity\/","name":"Cristina Cosi: Expression of c-fos mRNA is an indirect marker of neuronal activity","isPartOf":{"@id":"https:\/\/www.kolabtree.com\/blog\/#website"},"datePublished":"2021-05-20T04:22:28+00:00","dateModified":"2021-05-20T04:40:52+00:00","description":"Neuroscientist Cristina Cosi's research publication on the expression of c-fos mRNA as an indirect marker of neuronal activity.","breadcrumb":{"@id":"https:\/\/www.kolabtree.com\/blog\/de\/cristina-cosi-expression-ofcfos-mrna-is-an-indirect-marker-of-neuronal-activity\/#breadcrumb"},"inLanguage":"de","potentialAction":[{"@type":"ReadAction","target":["https:\/\/www.kolabtree.com\/blog\/de\/cristina-cosi-expression-ofcfos-mrna-is-an-indirect-marker-of-neuronal-activity\/"]}]},{"@type":"BreadcrumbList","@id":"https:\/\/www.kolabtree.com\/blog\/de\/cristina-cosi-expression-ofcfos-mrna-is-an-indirect-marker-of-neuronal-activity\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/www.kolabtree.com\/blog\/"},{"@type":"ListItem","position":2,"name":"Cristina Cosi: Expression of c-fos mRNA is an indirect marker of neuronal activity"}]},{"@type":"WebSite","@id":"https:\/\/www.kolabtree.com\/blog\/#website","url":"https:\/\/www.kolabtree.com\/blog\/","name":"The Kolabtree Blog","description":"Expert Views on Science, Innovation and Product Development","publisher":{"@id":"https:\/\/www.kolabtree.com\/blog\/#organization"},"potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/www.kolabtree.com\/blog\/?s={search_term_string}"},"query-input":"required name=search_term_string"}],"inLanguage":"de"},{"@type":"Organization","@id":"https:\/\/www.kolabtree.com\/blog\/#organization","name":"Kolabtree","url":"https:\/\/www.kolabtree.com\/blog\/","logo":{"@type":"ImageObject","inLanguage":"de","@id":"https:\/\/www.kolabtree.com\/blog\/#\/schema\/logo\/image\/","url":"","contentUrl":"","caption":"Kolabtree"},"image":{"@id":"https:\/\/www.kolabtree.com\/blog\/#\/schema\/logo\/image\/"},"sameAs":["https:\/\/www.facebook.com\/kolabtree","https:\/\/twitter.com\/kolabtree","https:\/\/instagram.com\/kolabtree","https:\/\/www.linkedin.com\/company\/kolabtree","https:\/\/en.m.wikipedia.org\/wiki\/Kolabtree"]},{"@type":"Person","@id":"https:\/\/www.kolabtree.com\/blog\/#\/schema\/person\/81992f5863a1b06d132a47822e7b4400","name":"Ramya Sriram","image":{"@type":"ImageObject","inLanguage":"de","@id":"https:\/\/www.kolabtree.com\/blog\/#\/schema\/person\/image\/","url":"https:\/\/secure.gravatar.com\/avatar\/8100b45c960ebbbbe420e8b3f250515f?s=96&d=mm&r=g","contentUrl":"https:\/\/secure.gravatar.com\/avatar\/8100b45c960ebbbbe420e8b3f250515f?s=96&d=mm&r=g","caption":"Ramya Sriram"},"description":"Ramya Sriram manages digital content and communications at Kolabtree (kolabtree.com), the world's largest freelancing platform for scientists. She has over a decade of experience in publishing, advertising and digital content creation.","url":"https:\/\/www.kolabtree.com\/blog\/de\/author\/ramyas\/"}]}},"amp_enabled":true,"_links":{"self":[{"href":"https:\/\/www.kolabtree.com\/blog\/de\/wp-json\/wp\/v2\/posts\/9141"}],"collection":[{"href":"https:\/\/www.kolabtree.com\/blog\/de\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.kolabtree.com\/blog\/de\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.kolabtree.com\/blog\/de\/wp-json\/wp\/v2\/users\/12"}],"replies":[{"embeddable":true,"href":"https:\/\/www.kolabtree.com\/blog\/de\/wp-json\/wp\/v2\/comments?post=9141"}],"version-history":[{"count":2,"href":"https:\/\/www.kolabtree.com\/blog\/de\/wp-json\/wp\/v2\/posts\/9141\/revisions"}],"predecessor-version":[{"id":9146,"href":"https:\/\/www.kolabtree.com\/blog\/de\/wp-json\/wp\/v2\/posts\/9141\/revisions\/9146"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.kolabtree.com\/blog\/de\/wp-json\/wp\/v2\/media\/9147"}],"wp:attachment":[{"href":"https:\/\/www.kolabtree.com\/blog\/de\/wp-json\/wp\/v2\/media?parent=9141"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.kolabtree.com\/blog\/de\/wp-json\/wp\/v2\/categories?post=9141"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.kolabtree.com\/blog\/de\/wp-json\/wp\/v2\/tags?post=9141"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}